Stromal cells expressing ephrin-B2 promote the growth and sprouting of ephrin-B2+ endothelial cells

Xiu Qin Zhang, Nobuyuki Takakura, Yuichi Oike, Tomohisa Inada, Nicholas W. Gale, George D. Yancopoulos, Toshio Suda

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Ephrin-B2 is a transmembrane ligand that is specifically expressed on arterial endothelial cells (ECs) and surrounding cells and interacts with multiple EphB class receptors. Conversely, EphB4, a specific receptor for ephrin-B2, is expressed on venous ECs, and both ephrin-B2 and EphB4 play essential roles in vascular development. The bidirectional signals between EphB4 and ephrin-B2 are thought to be specific for the interaction between arteries and veins and to regulate cell mixing and the making of particular boundaries. However, the molecular mechanism during vasculogenesis and angiogenesis remains unclear. Manipulative functional studies were performed on these proteins in an endothelial cell system. Using in vitro stromal cells (OP9 cells) and a paraaortic splanchnopleura (P-Sp) coculture system, these studies found that the stromal cells expressing ephrin-B2 promoted vascular network formation and ephrin-B2+ EC proliferation and that they also induced the recruitment and proliferation of α-smooth muscle actin (α-SMA)-positive cells. Stromal cells expressing EphB4 inhibited vascular network formation, ephrin-B2+ EC proliferation, and α-SMA+ cell recruitment and proliferation. Thus, these data suggest that ephrin-B2 and EphB4 mediate reciprocal interactions between arterial and venous ECs and surrounding cells to form each characteristic vessel.

Original languageEnglish
Pages (from-to)1028-1037
Number of pages10
JournalBlood
Volume98
Issue number4
DOIs
Publication statusPublished - 2001 Aug 15

Fingerprint

Ephrin-B2
Endothelial cells
Stromal Cells
Endothelial Cells
Cells
Growth
Blood Vessels
Cell Proliferation
Cell proliferation
Eph Family Receptors
Coculture Techniques
Smooth Muscle
Muscle
Actins
Veins
Arteries
Ligands

ASJC Scopus subject areas

  • Hematology

Cite this

Zhang, X. Q., Takakura, N., Oike, Y., Inada, T., Gale, N. W., Yancopoulos, G. D., & Suda, T. (2001). Stromal cells expressing ephrin-B2 promote the growth and sprouting of ephrin-B2+ endothelial cells. Blood, 98(4), 1028-1037. https://doi.org/10.1182/blood.V98.4.1028

Stromal cells expressing ephrin-B2 promote the growth and sprouting of ephrin-B2+ endothelial cells. / Zhang, Xiu Qin; Takakura, Nobuyuki; Oike, Yuichi; Inada, Tomohisa; Gale, Nicholas W.; Yancopoulos, George D.; Suda, Toshio.

In: Blood, Vol. 98, No. 4, 15.08.2001, p. 1028-1037.

Research output: Contribution to journalArticle

Zhang, XQ, Takakura, N, Oike, Y, Inada, T, Gale, NW, Yancopoulos, GD & Suda, T 2001, 'Stromal cells expressing ephrin-B2 promote the growth and sprouting of ephrin-B2+ endothelial cells', Blood, vol. 98, no. 4, pp. 1028-1037. https://doi.org/10.1182/blood.V98.4.1028
Zhang XQ, Takakura N, Oike Y, Inada T, Gale NW, Yancopoulos GD et al. Stromal cells expressing ephrin-B2 promote the growth and sprouting of ephrin-B2+ endothelial cells. Blood. 2001 Aug 15;98(4):1028-1037. https://doi.org/10.1182/blood.V98.4.1028
Zhang, Xiu Qin ; Takakura, Nobuyuki ; Oike, Yuichi ; Inada, Tomohisa ; Gale, Nicholas W. ; Yancopoulos, George D. ; Suda, Toshio. / Stromal cells expressing ephrin-B2 promote the growth and sprouting of ephrin-B2+ endothelial cells. In: Blood. 2001 ; Vol. 98, No. 4. pp. 1028-1037.
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