Stromal macrophage expressing CD204 is associated with tumor aggressiveness in lung adenocarcinoma

Yoichi Ohtaki, Genichiro Ishii, Kanji Nagai, Satoshi Ashimine, Takeshi Kuwata, Tomoyuki Hishida, Mitsuyo Nishimura, Junji Yoshida, Izumi Takeyoshi, Atsushi Ochiai

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Background: Tumor tissue is composed of variable numbers of cancer cells and stromal cells, and tumor-associated macrophages are recruited into cancer-induced stroma and produce a specific microenvironment. Alternatively, activated macrophages (M2 phenotype) are known to be related to tumor progression and outcome, and CD204 has been reported to be expressed in M2 macrophages in some tumors. Methods: To investigate whether CD204-positive macrophages reflect tumor aggressiveness in adenocarcinoma of the lung, we investigated the relationships between the numbers of CD204-positive stromal macrophages and both clinicopathological features and outcome in 170 consecutive resected cases. We also examined the relationships between the numbers of CD204-positive macrophages and the expression levels of cytokines involved in the migration and differentiation of M2 macrophages. Results: The numbers of CD204-positive macrophages were significantly correlated with several prognostic factors. The log-rank test showed a significant association between the numbers of CD204-positive macrophages and a poor outcome (p = 0.0073), whereas the numbers of macrophages expressing CD68, a pan-macrophage/monocyte marker, were of marginal prognostic significance (p = 0.0789). We evaluated associations between the levels of expression of the cytokines IL-6, IL-10, IL-12a, IL-12b, M-colony-stimulating factor, IFN-gamma-., and monocyte chemoattractant protein-1 in cancer tissue and the numbers of CD204-positive macrophages. The expression levels of IL-10 and monocyte chemoattractant protein-1, which are involved in differentiation, accumulation, and migration of M2 macrophages, were significantly correlated with the numbers of CD204-positive macrophages (p = 0.031 and p = 0.031, respectively). Conclusion: These findings demonstrated that CD204-positive macrophages clearly reflect the tumor-promoting phenotype of tumor-associated macrophages in lung adenocarcinoma.

Original languageEnglish
Pages (from-to)1507-1515
Number of pages9
JournalJournal of Thoracic Oncology
Volume5
Issue number10
DOIs
Publication statusPublished - 2010 Oct
Externally publishedYes

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Macrophages
Neoplasms
Adenocarcinoma of lung
Chemokine CCL2
Interleukin-10
Cytokines
Phenotype
Colony-Stimulating Factors
Stromal Cells
Monocytes
Interleukin-6
Cell Count

Keywords

  • CD204
  • Lung cancer
  • Macrophage
  • Stroma
  • Tumor-associated macrophages (TAMs)

ASJC Scopus subject areas

  • Oncology
  • Pulmonary and Respiratory Medicine

Cite this

Stromal macrophage expressing CD204 is associated with tumor aggressiveness in lung adenocarcinoma. / Ohtaki, Yoichi; Ishii, Genichiro; Nagai, Kanji; Ashimine, Satoshi; Kuwata, Takeshi; Hishida, Tomoyuki; Nishimura, Mitsuyo; Yoshida, Junji; Takeyoshi, Izumi; Ochiai, Atsushi.

In: Journal of Thoracic Oncology, Vol. 5, No. 10, 10.2010, p. 1507-1515.

Research output: Contribution to journalArticle

Ohtaki, Y, Ishii, G, Nagai, K, Ashimine, S, Kuwata, T, Hishida, T, Nishimura, M, Yoshida, J, Takeyoshi, I & Ochiai, A 2010, 'Stromal macrophage expressing CD204 is associated with tumor aggressiveness in lung adenocarcinoma', Journal of Thoracic Oncology, vol. 5, no. 10, pp. 1507-1515. https://doi.org/10.1097/JTO.0b013e3181eba692
Ohtaki, Yoichi ; Ishii, Genichiro ; Nagai, Kanji ; Ashimine, Satoshi ; Kuwata, Takeshi ; Hishida, Tomoyuki ; Nishimura, Mitsuyo ; Yoshida, Junji ; Takeyoshi, Izumi ; Ochiai, Atsushi. / Stromal macrophage expressing CD204 is associated with tumor aggressiveness in lung adenocarcinoma. In: Journal of Thoracic Oncology. 2010 ; Vol. 5, No. 10. pp. 1507-1515.
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T1 - Stromal macrophage expressing CD204 is associated with tumor aggressiveness in lung adenocarcinoma

AU - Ohtaki, Yoichi

AU - Ishii, Genichiro

AU - Nagai, Kanji

AU - Ashimine, Satoshi

AU - Kuwata, Takeshi

AU - Hishida, Tomoyuki

AU - Nishimura, Mitsuyo

AU - Yoshida, Junji

AU - Takeyoshi, Izumi

AU - Ochiai, Atsushi

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KW - CD204

KW - Lung cancer

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KW - Stroma

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