Structural analysis of glycosphingolipid analogues obtained by the saccharide primer method using CE-ESI-MS

Xingyu Zhu, Kenichi Hatanaka, Tatsuya Yamagata, Toshinori Sato

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A glycosphingolipid analogue (12-azidododecyl β-lactoside) as a saccharide primer has been shown to be useful for the synthesis of oligosaccharide libraries by mammalian cells. In the present study, CE-ESI-MS was employed to elucidate the structure of glycosphingolipid analogues derived from 12-azidododecyl b-lactoside (Lac-C12N3) by mammalian cells. MDCK cells and COLO201 cells were cultured with Lac-C12N3, and the glycosylated products secreted into the medium were collected and separated into acidic and neutral products by column chromatography. The acidic products could be directly analyzed by CEESI-MS, while the neutral products were converted to anionic derivatives via a reaction with propiolic acid. With this method, it was possible to analyze both acidic and neutral products glycosylated by MDCK cells and COLO201 cells at high sensitivity.

Original languageEnglish
Pages (from-to)3519-3526
Number of pages8
JournalElectrophoresis
Volume30
Issue number20
DOIs
Publication statusPublished - 2009

Fingerprint

Glycosphingolipids
Structural analysis
Madin Darby Canine Kidney Cells
Cells
Column chromatography
Oligosaccharides
Derivatives
Chromatography
Cultured Cells
12-azidododecyl lactoside
propiolic acid
lactosides

Keywords

  • CE-ESI-MS
  • Neutral carbohydrate
  • Saccharide primer

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Structural analysis of glycosphingolipid analogues obtained by the saccharide primer method using CE-ESI-MS. / Zhu, Xingyu; Hatanaka, Kenichi; Yamagata, Tatsuya; Sato, Toshinori.

In: Electrophoresis, Vol. 30, No. 20, 2009, p. 3519-3526.

Research output: Contribution to journalArticle

Zhu, Xingyu ; Hatanaka, Kenichi ; Yamagata, Tatsuya ; Sato, Toshinori. / Structural analysis of glycosphingolipid analogues obtained by the saccharide primer method using CE-ESI-MS. In: Electrophoresis. 2009 ; Vol. 30, No. 20. pp. 3519-3526.
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