TY - JOUR
T1 - Structural analysis of HTL and D14 proteins reveals the basis for ligand selectivity in Striga
AU - Xu, Yuqun
AU - Miyakawa, Takuya
AU - Nosaki, Shohei
AU - Nakamura, Akira
AU - Lyu, Ying
AU - Nakamura, Hidemitsu
AU - Ohto, Umeharu
AU - Ishida, Hanako
AU - Shimizu, Toshiyuki
AU - Asami, Tadao
AU - Tanokura, Masaru
N1 - Funding Information:
The synchrotron radiation experiments were performed on beamlines AR-NW12A, BL5A, and BL17A at the Photon Factory with the approval of the High Energy Accelerator Research Organization (Proposal No. 2015R-25). This work was supported by the Platform for Drug Discovery, Informatics, and Structural Life Science from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT) (M.T.), the Core Research for Evolutional Science and Technology (CREST) Program of the Japan Science and Technology Agency (JST) (T.A.), and a Grant-in-Aid for Scientific Research in Innovative Areas (JP17H05835 (T.M.) and JP18H04608 (H.N.)) from the Japan Society for the Promotion of Science (JSPS) and JSPS KAKENHI (JP15H05621 (T.M.) and JP26520303 (H.N.)). We thank Ikuo Takahashi for his help with the activity measurements.
Publisher Copyright:
© 2018, The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - HYPOSENSITIVE TO LIGHT (HTL) and DWARF14 (D14) mediate the perception of karrikin and strigolactone, which stimulates germination of the parasitic weed Striga. However, their role in parasitic seeds is poorly understood, and the basis for their differing responsiveness remains unclear. Here, we show that Striga hermonthica HTL proteins (ShHTLs) in ‘conserved’ and ‘intermediate’ clades are able to bind karrikin. The ‘divergent’ clade is able to hydrolyze strigolactone. Unexpectedly, we find that ShD14 is also capable of hydrolyzing strigolactone. Through comparative analysis of ShHTLs and ShD14 crystal structures, we provide insights into the basis for their selectivity. Moreover, we show that both ShD14 and divergent clade ShHTLs, but not conserved and intermediate clade ShHTLs, can interact with the putative downstream signaling component ShMAX2 in the presence of the synthetic strigolactone, rac-GR24. These findings provide insight into how strigolactone is perceived and how ligand specificity is determined.
AB - HYPOSENSITIVE TO LIGHT (HTL) and DWARF14 (D14) mediate the perception of karrikin and strigolactone, which stimulates germination of the parasitic weed Striga. However, their role in parasitic seeds is poorly understood, and the basis for their differing responsiveness remains unclear. Here, we show that Striga hermonthica HTL proteins (ShHTLs) in ‘conserved’ and ‘intermediate’ clades are able to bind karrikin. The ‘divergent’ clade is able to hydrolyze strigolactone. Unexpectedly, we find that ShD14 is also capable of hydrolyzing strigolactone. Through comparative analysis of ShHTLs and ShD14 crystal structures, we provide insights into the basis for their selectivity. Moreover, we show that both ShD14 and divergent clade ShHTLs, but not conserved and intermediate clade ShHTLs, can interact with the putative downstream signaling component ShMAX2 in the presence of the synthetic strigolactone, rac-GR24. These findings provide insight into how strigolactone is perceived and how ligand specificity is determined.
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U2 - 10.1038/s41467-018-06452-2
DO - 10.1038/s41467-018-06452-2
M3 - Article
C2 - 30258184
AN - SCOPUS:85054067222
SN - 2041-1723
VL - 9
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 3947
ER -