Structural analysis of p28 adult T-cell leukaemia-associated antigen.

T. Iino, K. Takeuchi, S. H. Nam, Haruhiko Siomi, H. Sabe, N. Kobayashi, M. Hatanaka

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The 28,000 mol. wt. polypeptide (p28) of adult T-cell leukaemia-associated antigen encoded by the 24S defective human T-cell leukaemia virus (HTLV-I) is associated with protein kinase activity. We have determined the nucleotide sequence of this defective HTLV-I provirus and found that it contains a portion of the gag gene (p19 and part of p24), the pX region, and two long terminal repeats, one at each end. The predicted p28 gag-pX fused protein consists of 190 amino acids and its mol. wt. was calculated as 21,055. The results of peptide mapping analysis showing that p28 contains p19 supported the nucleotide sequence data. That p28 was encoded by this defective provirus was also demonstrated by transient expression of p28 polypeptide in COS 7 cells transfected with a recombinant plasmid containing a simian virus 40 early promoter and the p28-coding region of the 24S HTLV-I.

Original languageEnglish
JournalThe Journal of general virology
Volume67
Publication statusPublished - 1986 Jul
Externally publishedYes

Fingerprint

Deltaretrovirus
Proviruses
Adult T Cell Leukemia Lymphoma
gag Genes
Peptides
Human T-lymphotropic virus 1
Peptide Mapping
Simian virus 40
Terminal Repeat Sequences
COS Cells
Protein Kinases
Plasmids
Amino Acids
Proteins
thymus-leukemia antigens

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Iino, T., Takeuchi, K., Nam, S. H., Siomi, H., Sabe, H., Kobayashi, N., & Hatanaka, M. (1986). Structural analysis of p28 adult T-cell leukaemia-associated antigen. The Journal of general virology, 67.

Structural analysis of p28 adult T-cell leukaemia-associated antigen. / Iino, T.; Takeuchi, K.; Nam, S. H.; Siomi, Haruhiko; Sabe, H.; Kobayashi, N.; Hatanaka, M.

In: The Journal of general virology, Vol. 67, 07.1986.

Research output: Contribution to journalArticle

Iino, T, Takeuchi, K, Nam, SH, Siomi, H, Sabe, H, Kobayashi, N & Hatanaka, M 1986, 'Structural analysis of p28 adult T-cell leukaemia-associated antigen.', The Journal of general virology, vol. 67.
Iino, T. ; Takeuchi, K. ; Nam, S. H. ; Siomi, Haruhiko ; Sabe, H. ; Kobayashi, N. ; Hatanaka, M. / Structural analysis of p28 adult T-cell leukaemia-associated antigen. In: The Journal of general virology. 1986 ; Vol. 67.
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