Structural and functional analysis of the intrinsic inhibitor subunit ε of F1-ATPase from photosynthetic organisms

Hiromasa Yagi, Hiroki Konno, Tomoe Murakami-Fuse, Atsuko Isu, Tomotaka Oroguchi, Hideo Akutsu, Mitsunori Ikeguchi, Toru Hisabori

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The ε subunit, a small subunit located in the F1 domain of ATP synthase and comprising two distinct domains, an N-terminal β-sandwich structure and a C-terminal α-helical region, serves as an intrinsic inhibitor of ATP hydrolysis activity. This inhibitory function is especially important in photosynthetic organisms as the enzyme cannot synthesize ATP in the dark, but may catalyse futile ATP hydrolysis reactions. To understand the structure-function relationship of this subunit in F1 from photosynthetic organisms, we solved the NMR structure of the ε subunit of ATP synthase obtained from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1, and examined the flexibility of the C-terminal domains usingmolecular dynamics simulations. In addition, we revealed the significance of the C-terminal α-helical region of the ε subunit in determining the binding affinity to the complex based on the assessment of the inhibition of ATPase activity by the cyanobacterial ε subunit and the chimaeric subunits composed of the N-terminal domain from the cyanobacterium and the C-terminal domain from spinach. The differences observed in the structural and biochemical properties of chloroplast and bacterial ε subunits explains the distinctive characteristics of the ε subunits in the ATPase complex of the photosynthetic organism.

Original languageEnglish
Pages (from-to)85-94
Number of pages10
JournalBiochemical Journal
Volume425
Issue number1
DOIs
Publication statusPublished - 2010 Jan 1
Externally publishedYes

Fingerprint

Functional analysis
Proton-Translocating ATPases
Structural analysis
Adenosine Triphosphate
Cyanobacteria
Adenosine Triphosphatases
Hydrolysis
Photosynthetic Reaction Center Complex Proteins
Spinacia oleracea
Sandwich structures
Chloroplasts
Nuclear magnetic resonance
Computer simulation
Enzymes

Keywords

  • ε subunit
  • Cyanobacterium
  • F-ATPase
  • Thermosyne-chococcus elongatus

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Structural and functional analysis of the intrinsic inhibitor subunit ε of F1-ATPase from photosynthetic organisms. / Yagi, Hiromasa; Konno, Hiroki; Murakami-Fuse, Tomoe; Isu, Atsuko; Oroguchi, Tomotaka; Akutsu, Hideo; Ikeguchi, Mitsunori; Hisabori, Toru.

In: Biochemical Journal, Vol. 425, No. 1, 01.01.2010, p. 85-94.

Research output: Contribution to journalArticle

Yagi, H, Konno, H, Murakami-Fuse, T, Isu, A, Oroguchi, T, Akutsu, H, Ikeguchi, M & Hisabori, T 2010, 'Structural and functional analysis of the intrinsic inhibitor subunit ε of F1-ATPase from photosynthetic organisms', Biochemical Journal, vol. 425, no. 1, pp. 85-94. https://doi.org/10.1042/BJ20091247
Yagi, Hiromasa ; Konno, Hiroki ; Murakami-Fuse, Tomoe ; Isu, Atsuko ; Oroguchi, Tomotaka ; Akutsu, Hideo ; Ikeguchi, Mitsunori ; Hisabori, Toru. / Structural and functional analysis of the intrinsic inhibitor subunit ε of F1-ATPase from photosynthetic organisms. In: Biochemical Journal. 2010 ; Vol. 425, No. 1. pp. 85-94.
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