Structural and functional identification of two distinct inspiratory neuronal populations at the level of the phrenic nucleus in the rat cervical spinal cord

Yoshio Shinozaki, Shigefumi Yokota, Fumikazu Miwakeichi, Mieczyslaw Pokorski, Ryoma Aoyama, Kentaro Fukuda, Hideaki Yoshida, Yoshiaki Toyama, Masaya Nakamura, Yasumasa Okada

Research output: Contribution to journalArticle

Abstract

The diaphragm is driven by phrenic motoneurons that are located in the cervical spinal cord. Although the anatomical location of the phrenic nucleus and the function of phrenic motoneurons at a single cellular level have been extensively analyzed, the spatiotemporal dynamics of phrenic motoneuron group activity have not been fully elucidated. In the present study, we analyzed the functional and structural characteristics of respiratory neuron population in the cervical spinal cord at the level of the phrenic nucleus by voltage imaging, together with histological analysis of neuronal and astrocytic distribution in the cervical spinal cord. We found spatially distinct two cellular populations that exhibited synchronized inspiratory activity on the transversely cut plane at C4–C5 levels and on the ventral surface of the mid cervical spinal cord in the isolated brainstem–spinal cord preparation of the neonatal rat. Inspiratory activity of one group emerged in the central portion of the ventral horn that corresponded to the central motor column, and the other appeared in the medial portion of the ventral horn that corresponded to the medial motor column. We identified by retrogradely labeling study that the anatomical distributions of phrenic and scalene motoneurons coincided with optically detected central and medial motor regions, respectively. Furthermore, we anatomically demonstrated closely located features of putative motoneurons, interneurons and astrocytes in these regions. Collectively, we report that phrenic and scalene motoneuron populations show synchronized inspiratory activities with distinct anatomical locations in the mid cervical spinal cord.

Original languageEnglish
JournalBrain Structure and Function
DOIs
Publication statusAccepted/In press - 2018 Jan 1

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Motor Neurons
Diaphragm
Population
Horns
Interneurons
Cervical Cord
Astrocytes
Neurons

Keywords

  • Astrocyte
  • Cervical spinal cord
  • Interneuron
  • Phrenic motoneuron
  • Phrenic nucleus
  • Respiratory control
  • Scalene motoneuron
  • Voltage imaging

ASJC Scopus subject areas

  • Anatomy
  • Neuroscience(all)
  • Histology

Cite this

Structural and functional identification of two distinct inspiratory neuronal populations at the level of the phrenic nucleus in the rat cervical spinal cord. / Shinozaki, Yoshio; Yokota, Shigefumi; Miwakeichi, Fumikazu; Pokorski, Mieczyslaw; Aoyama, Ryoma; Fukuda, Kentaro; Yoshida, Hideaki; Toyama, Yoshiaki; Nakamura, Masaya; Okada, Yasumasa.

In: Brain Structure and Function, 01.01.2018.

Research output: Contribution to journalArticle

Shinozaki, Yoshio ; Yokota, Shigefumi ; Miwakeichi, Fumikazu ; Pokorski, Mieczyslaw ; Aoyama, Ryoma ; Fukuda, Kentaro ; Yoshida, Hideaki ; Toyama, Yoshiaki ; Nakamura, Masaya ; Okada, Yasumasa. / Structural and functional identification of two distinct inspiratory neuronal populations at the level of the phrenic nucleus in the rat cervical spinal cord. In: Brain Structure and Function. 2018.
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