Structural and immunochemical studies of teichoic acid of Listeria monocytogenes

Ken Ichi Kamisango, Hideji Fujii, Hiroyuki Okumura, Ikuo Saiki, Yoshio Araki, Yuichi Yamamura, Ichiro Azuma

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44 Citations (Scopus)

Abstract

An immunologically active teichoic acid component was isolated from the cell wall of Listeria monocytogenes strain EGD. The teichoic acid component, accounting for about 20% of the weight of cell wall, contained N-acetylglucosamine, rhamnose, ribitol, and phosphorus in a molar ratio of 0.95 : 1.0 : 0.97 : 0.98. The molecular weight of the teichoic acid chain was about 120,000 as analyzed by gel filtration. The probable structure was deduced from the results of methylation analysis, Smith degradation, and proton magnetic resonance spectrometry of the teichoic acid, together with the characterization of fragments obtained by treatment with hydrofluoric acid, as follows:Inhibition testing with monosaccharide and fragments obtained from HF treatment of Listeria teichoic acid in the quantitative precipitin reaction suggested that the rhamnose residue is a major antigenic determinant.

Original languageEnglish
Pages (from-to)1401-1409
Number of pages9
JournalJournal of biochemistry
Volume93
Issue number5
DOIs
Publication statusPublished - 1983
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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