Structural basis of the KcsA K+ channel and agitoxin2 pore-blocking toxin interaction by using the transferred cross-saturation method

Koh Takeuchi, Mariko Yokogawa, Tomoki Matsuda, Mariko Sugai, Seiko Kawano, Toshiyuki Kohno, Haruki Nakamura, Hideo Takahashi, Ichio Shimada

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We have determined the binding site on agitoxin2 (AgTx2) to the KcsA K + channel by a transferred cross-saturation (TCS) experiment. The residues significantly affected in the TCS experiments formed a contiguous surface on AgTx2, and substitutions of the surface residues decreased the binding affinity to the KcsA K+ channel. Based on properties of the AgTx2 binding site with the KcsA K+ channel, we present a surface motif that is observed in pore-blocking toxins affecting the K+ channel. Furthermore, we also explain the structural basis of the specificity of the K+ channel to the toxins. The TCS method utilized here is applicable not only for the channels, which are complexed with other inhibitors, but also with a variety of regulatory molecules, and provides important information about their interface in solution.

Original languageEnglish
Pages (from-to)1381-1392
Number of pages12
JournalStructure
Volume11
Issue number11
DOIs
Publication statusPublished - 2003 Nov
Externally publishedYes

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ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

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Structural basis of the KcsA K+ channel and agitoxin2 pore-blocking toxin interaction by using the transferred cross-saturation method. / Takeuchi, Koh; Yokogawa, Mariko; Matsuda, Tomoki; Sugai, Mariko; Kawano, Seiko; Kohno, Toshiyuki; Nakamura, Haruki; Takahashi, Hideo; Shimada, Ichio.

In: Structure, Vol. 11, No. 11, 11.2003, p. 1381-1392.

Research output: Contribution to journalArticle

Takeuchi, K, Yokogawa, M, Matsuda, T, Sugai, M, Kawano, S, Kohno, T, Nakamura, H, Takahashi, H & Shimada, I 2003, 'Structural basis of the KcsA K+ channel and agitoxin2 pore-blocking toxin interaction by using the transferred cross-saturation method', Structure, vol. 11, no. 11, pp. 1381-1392. https://doi.org/10.1016/j.str.2003.10.003
Takeuchi, Koh ; Yokogawa, Mariko ; Matsuda, Tomoki ; Sugai, Mariko ; Kawano, Seiko ; Kohno, Toshiyuki ; Nakamura, Haruki ; Takahashi, Hideo ; Shimada, Ichio. / Structural basis of the KcsA K+ channel and agitoxin2 pore-blocking toxin interaction by using the transferred cross-saturation method. In: Structure. 2003 ; Vol. 11, No. 11. pp. 1381-1392.
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AU - Kawano, Seiko

AU - Kohno, Toshiyuki

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AU - Takahashi, Hideo

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