Structural basis underlying the dual gate properties of KcsA

Shunsuke Imai, Masanori Osawa, Koh Takeuchi, Ichio Shimada

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

KcsA is a prokaryotic pH-dependent potassium (K) channel. Its activation, by a decrease in the intracellular pH, is coupled with its subsequent inactivation, but the underlying mechanisms remain elusive. Here, we have investigated the conformational changes and equilibrium of KcsA by using solution NMR spectroscopy. Controlling the temperature and pH of KcsA samples produced three distinct methyl-TROSY and NOESY spectra, corresponding to the resting, activated, and inactivated states. The pH-dependence of the signals from the extracellular side was affected by the mutation of H25 on the intracellular side, indicating the coupled conformational changes of the extracellular and intracellular gates. K+ titration and NOE experiments revealed that the inactivated state was obtained by the replacement of K+ with H2O, which may interfere with the K +-permeation. This structural basis of the activation-coupled inactivation is closely related to the C-type inactivation of other K channels.

Original languageEnglish
Pages (from-to)6216-6221
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number14
DOIs
Publication statusPublished - 2010 Apr 6
Externally publishedYes

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Potassium Channels
Magnetic Resonance Spectroscopy
Mutation
Temperature

Keywords

  • Gating
  • Inactivation
  • Potassium channel
  • Solution NMR

ASJC Scopus subject areas

  • General

Cite this

Structural basis underlying the dual gate properties of KcsA. / Imai, Shunsuke; Osawa, Masanori; Takeuchi, Koh; Shimada, Ichio.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 14, 06.04.2010, p. 6216-6221.

Research output: Contribution to journalArticle

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