Structural characterization of y29f mutant of thermoglobin from a hyperthermophilic bacterium aquifex aeolicus

Norifumi Muraki, Kouta Takeda, Dayeon Nam, Megumi Muraki, Shigetoshi Aono

Research output: Contribution to journalArticlepeer-review

Abstract

We have determined the crystal structure of thermoglobin (AaTgb) from a hyperthermophilic bacterium Aquifex aeolicus. Tyrosine and glutamine at the B10 and E7 position, respectively, are conserved in AaTgb as are the case of single domain hemoglobins (sdHbs). While the binding affinity of O2 or CO is affected by the replacement of Tyr29 in the distal heme pocket, wild type and Y29F variant of AaTgb show a similar binding affinity of imidazole.

Original languageEnglish
Article number200879
JournalChemistry Letters
Volume50
Issue number4
DOIs
Publication statusPublished - 2021
Externally publishedYes

Keywords

  • Hemeprotein
  • Single domain hemoglobin
  • Thermoglobin

ASJC Scopus subject areas

  • Chemistry(all)

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