Structural differences in 5′-flanking regions of rat cytochrome P-450aldo and P-45011β genes

Kuniaki Mukai, Michiyo Imai, Hideo Shimada, Yukiko Okada, Tadashi Ogishima, Yuzuru Ishimura

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Two rat genomic clones, one for cytochrome P-450aldo and the other for P45011β, were isolated and characterized. The two genes, encoding structurally homologous proteins, were closely similar in their intron-exon organizations. Their 5′-flanking regions, however, contained only a few homologous regions. A putative cyclic AMP responsive element, TGACGTGA, was found in the P-450aldo gene, but this sequence was altered at two positions in the P-45011β gene. S1 nuclease protection assay revealed a single transcription initiation site for the P450aldo gene, while multiple sites were found for the P-45011β gene. These results suggest that transcriptional regulation of the rat P-450aldo and P-45011β genes is due to differences in the sequences of their 5′-flanking regions.

Original languageEnglish
Pages (from-to)1187-1193
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume180
Issue number3
DOIs
Publication statusPublished - 1991 Nov 14

Fingerprint

5' Flanking Region
Cytochromes
Rats
Genes
Nuclease Protection Assays
Gene encoding
Transcription Initiation Site
Cyclic AMP
Introns
Exons
Assays
Clone Cells
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Structural differences in 5′-flanking regions of rat cytochrome P-450aldo and P-45011β genes. / Mukai, Kuniaki; Imai, Michiyo; Shimada, Hideo; Okada, Yukiko; Ogishima, Tadashi; Ishimura, Yuzuru.

In: Biochemical and Biophysical Research Communications, Vol. 180, No. 3, 14.11.1991, p. 1187-1193.

Research output: Contribution to journalArticle

Mukai, Kuniaki ; Imai, Michiyo ; Shimada, Hideo ; Okada, Yukiko ; Ogishima, Tadashi ; Ishimura, Yuzuru. / Structural differences in 5′-flanking regions of rat cytochrome P-450aldo and P-45011β genes. In: Biochemical and Biophysical Research Communications. 1991 ; Vol. 180, No. 3. pp. 1187-1193.
@article{5fb5793f04a845b893e2579fe718b711,
title = "Structural differences in 5′-flanking regions of rat cytochrome P-450aldo and P-45011β genes",
abstract = "Two rat genomic clones, one for cytochrome P-450aldo and the other for P45011β, were isolated and characterized. The two genes, encoding structurally homologous proteins, were closely similar in their intron-exon organizations. Their 5′-flanking regions, however, contained only a few homologous regions. A putative cyclic AMP responsive element, TGACGTGA, was found in the P-450aldo gene, but this sequence was altered at two positions in the P-45011β gene. S1 nuclease protection assay revealed a single transcription initiation site for the P450aldo gene, while multiple sites were found for the P-45011β gene. These results suggest that transcriptional regulation of the rat P-450aldo and P-45011β genes is due to differences in the sequences of their 5′-flanking regions.",
author = "Kuniaki Mukai and Michiyo Imai and Hideo Shimada and Yukiko Okada and Tadashi Ogishima and Yuzuru Ishimura",
year = "1991",
month = "11",
day = "14",
doi = "10.1016/S0006-291X(05)81321-X",
language = "English",
volume = "180",
pages = "1187--1193",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Structural differences in 5′-flanking regions of rat cytochrome P-450aldo and P-45011β genes

AU - Mukai, Kuniaki

AU - Imai, Michiyo

AU - Shimada, Hideo

AU - Okada, Yukiko

AU - Ogishima, Tadashi

AU - Ishimura, Yuzuru

PY - 1991/11/14

Y1 - 1991/11/14

N2 - Two rat genomic clones, one for cytochrome P-450aldo and the other for P45011β, were isolated and characterized. The two genes, encoding structurally homologous proteins, were closely similar in their intron-exon organizations. Their 5′-flanking regions, however, contained only a few homologous regions. A putative cyclic AMP responsive element, TGACGTGA, was found in the P-450aldo gene, but this sequence was altered at two positions in the P-45011β gene. S1 nuclease protection assay revealed a single transcription initiation site for the P450aldo gene, while multiple sites were found for the P-45011β gene. These results suggest that transcriptional regulation of the rat P-450aldo and P-45011β genes is due to differences in the sequences of their 5′-flanking regions.

AB - Two rat genomic clones, one for cytochrome P-450aldo and the other for P45011β, were isolated and characterized. The two genes, encoding structurally homologous proteins, were closely similar in their intron-exon organizations. Their 5′-flanking regions, however, contained only a few homologous regions. A putative cyclic AMP responsive element, TGACGTGA, was found in the P-450aldo gene, but this sequence was altered at two positions in the P-45011β gene. S1 nuclease protection assay revealed a single transcription initiation site for the P450aldo gene, while multiple sites were found for the P-45011β gene. These results suggest that transcriptional regulation of the rat P-450aldo and P-45011β genes is due to differences in the sequences of their 5′-flanking regions.

UR - http://www.scopus.com/inward/record.url?scp=0025833716&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025833716&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(05)81321-X

DO - 10.1016/S0006-291X(05)81321-X

M3 - Article

C2 - 1953771

AN - SCOPUS:0025833716

VL - 180

SP - 1187

EP - 1193

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -