Structural information on proteins obtainable from small-angle X-ray scattering with heavy-atom labeling. Application to solubilized bacteriorhodopsin

M. Kataoka, M. Nakasako, F. Tokunaga

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The heavy-atom labeling method is applied to small-angle X-ray scattering (SAXS) based on contrast variation in order to derive information on the inner structure of proteins. The formulation of the contrast variation is extended to express explicitly the contribution of the bound heavy atoms to SAXS. The present method reveals two important features concerning bound heavy atoms, namely their number and their distribution. The application of this method to solubilized bacteriorhodopsin (bR) with iodination revealed the following characteristics of structure. The number of bound iodine atoms is 20, indicating that the solubilized bR is composed of a trimer of bR molecules. The mean square distance between the iodine position and the center of the trimer is about 1660 Å2, indicating that the most active tyrosine residues are located near the outer rim of a bR trimer. The distribution of iodine atoms is symmetric about the center.

Original languageEnglish
Pages (from-to)355-362
Number of pages8
JournalJournal of Applied Crystallography
Volume21
Issue number4
DOIs
Publication statusPublished - 1988

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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