Abstract
mouse-Musashi-1 (m-Msi-1) is an RNA-binding protein, abundantly expressed in the developing mammalian central nervous system (CNS). m-Msi-1 contains two RNA recognition motifs (RRMs). In this study, we found that the N-terminal RRM of m-Msi-1 (MMA) binds strongly to poly(G) and weakly to poly(U) in a way similar to that of the full-length m-Msi-1 protein characterized previously. The C-terminal RRM of m-Msi-1 (MMB), however, does not bind to RNA. In addition, the circular dichroism (CD) spectra of the two RRMs showed that the α-helical content of MMA is significantly higher than that of MMB, indicating that some differences in the secondary structure may be responsible for the distinct RNA binding properties of MMA and MMB.
| Original language | English |
|---|---|
| Pages (from-to) | 21-27 |
| Number of pages | 7 |
| Journal | Gene |
| Volume | 186 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1997 Feb 20 |
| Externally published | Yes |
Fingerprint
Keywords
- α-helix
- β-sheet
- Circular dichroism
- Elav
- Musashi
- Northwestern blotting
- Secondary structure
- U1 snRNP A
ASJC Scopus subject areas
- Genetics
Cite this
Structural properties and RNA-binding activities of two RNA recognition motifs of a mouse neural RNA-binding protein, mouse-Musashi-1. / Kurihara, Yasuyuki; Nagata, Takashi; Imai, Takao; Hiwatashi, Ado; Horiuchi, Masataka; Sakakibara, Shin ichi; Katahira, Masato; Okano, Hideyuki; Uesugi, Seiichi.
In: Gene, Vol. 186, No. 1, 20.02.1997, p. 21-27.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Structural properties and RNA-binding activities of two RNA recognition motifs of a mouse neural RNA-binding protein, mouse-Musashi-1
AU - Kurihara, Yasuyuki
AU - Nagata, Takashi
AU - Imai, Takao
AU - Hiwatashi, Ado
AU - Horiuchi, Masataka
AU - Sakakibara, Shin ichi
AU - Katahira, Masato
AU - Okano, Hideyuki
AU - Uesugi, Seiichi
PY - 1997/2/20
Y1 - 1997/2/20
N2 - mouse-Musashi-1 (m-Msi-1) is an RNA-binding protein, abundantly expressed in the developing mammalian central nervous system (CNS). m-Msi-1 contains two RNA recognition motifs (RRMs). In this study, we found that the N-terminal RRM of m-Msi-1 (MMA) binds strongly to poly(G) and weakly to poly(U) in a way similar to that of the full-length m-Msi-1 protein characterized previously. The C-terminal RRM of m-Msi-1 (MMB), however, does not bind to RNA. In addition, the circular dichroism (CD) spectra of the two RRMs showed that the α-helical content of MMA is significantly higher than that of MMB, indicating that some differences in the secondary structure may be responsible for the distinct RNA binding properties of MMA and MMB.
AB - mouse-Musashi-1 (m-Msi-1) is an RNA-binding protein, abundantly expressed in the developing mammalian central nervous system (CNS). m-Msi-1 contains two RNA recognition motifs (RRMs). In this study, we found that the N-terminal RRM of m-Msi-1 (MMA) binds strongly to poly(G) and weakly to poly(U) in a way similar to that of the full-length m-Msi-1 protein characterized previously. The C-terminal RRM of m-Msi-1 (MMB), however, does not bind to RNA. In addition, the circular dichroism (CD) spectra of the two RRMs showed that the α-helical content of MMA is significantly higher than that of MMB, indicating that some differences in the secondary structure may be responsible for the distinct RNA binding properties of MMA and MMB.
KW - α-helix
KW - β-sheet
KW - Circular dichroism
KW - Elav
KW - Musashi
KW - Northwestern blotting
KW - Secondary structure
KW - U1 snRNP A
UR - http://www.scopus.com/inward/record.url?scp=0031579450&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031579450&partnerID=8YFLogxK
U2 - 10.1016/S0378-1119(96)00673-7
DO - 10.1016/S0378-1119(96)00673-7
M3 - Article
C2 - 9047340
AN - SCOPUS:0031579450
VL - 186
SP - 21
EP - 27
JO - Gene
JF - Gene
SN - 0378-1119
IS - 1
ER -