Structural properties and RNA-binding activities of two RNA recognition motifs of a mouse neural RNA-binding protein, mouse-Musashi-1

Yasuyuki Kurihara; Takashi Nagata; Takao Imai; Ado Hiwatashi; Masataka Horiuchi; Shin ichi Sakakibara; Masato Katahira; Hideyuki Okano; Seiichi Uesugi

doi:10.1016/S0378-1119(96)00673-7

Structural properties and RNA-binding activities of two RNA recognition motifs of a mouse neural RNA-binding protein, mouse-Musashi-1

Yasuyuki Kurihara, Takashi Nagata, Takao Imai, Ado Hiwatashi, Masataka Horiuchi, Shin ichi Sakakibara, Masato Katahira, Hideyuki Okano, Seiichi Uesugi

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

mouse-Musashi-1 (m-Msi-1) is an RNA-binding protein, abundantly expressed in the developing mammalian central nervous system (CNS). m-Msi-1 contains two RNA recognition motifs (RRMs). In this study, we found that the N-terminal RRM of m-Msi-1 (MMA) binds strongly to poly(G) and weakly to poly(U) in a way similar to that of the full-length m-Msi-1 protein characterized previously. The C-terminal RRM of m-Msi-1 (MMB), however, does not bind to RNA. In addition, the circular dichroism (CD) spectra of the two RRMs showed that the α-helical content of MMA is significantly higher than that of MMB, indicating that some differences in the secondary structure may be responsible for the distinct RNA binding properties of MMA and MMB.

Original language	English
Pages (from-to)	21-27
Number of pages	7
Journal	Gene
Volume	186
Issue number	1
DOIs	https://doi.org/10.1016/S0378-1119(96)00673-7
Publication status	Published - 1997 Feb 20
Externally published	Yes

Keywords

Circular dichroism
Elav
Musashi
Northwestern blotting
Secondary structure
U1 snRNP A
α-helix
β-sheet

ASJC Scopus subject areas

Genetics

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10.1016/S0378-1119(96)00673-7

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@article{bd0e768572474e87877c150e6292f663,

title = "Structural properties and RNA-binding activities of two RNA recognition motifs of a mouse neural RNA-binding protein, mouse-Musashi-1",

abstract = "mouse-Musashi-1 (m-Msi-1) is an RNA-binding protein, abundantly expressed in the developing mammalian central nervous system (CNS). m-Msi-1 contains two RNA recognition motifs (RRMs). In this study, we found that the N-terminal RRM of m-Msi-1 (MMA) binds strongly to poly(G) and weakly to poly(U) in a way similar to that of the full-length m-Msi-1 protein characterized previously. The C-terminal RRM of m-Msi-1 (MMB), however, does not bind to RNA. In addition, the circular dichroism (CD) spectra of the two RRMs showed that the α-helical content of MMA is significantly higher than that of MMB, indicating that some differences in the secondary structure may be responsible for the distinct RNA binding properties of MMA and MMB.",

keywords = "Circular dichroism, Elav, Musashi, Northwestern blotting, Secondary structure, U1 snRNP A, α-helix, β-sheet",

author = "Yasuyuki Kurihara and Takashi Nagata and Takao Imai and Ado Hiwatashi and Masataka Horiuchi and Sakakibara, {Shin ichi} and Masato Katahira and Hideyuki Okano and Seiichi Uesugi",

note = "Funding Information: The authors thank Mr. M. Miyauchi and M. Ohtsu for their skillful technical assistance, and Dr. M. Okabe for a kind help in preparing the manuscript. This work was supported by grants from the Japanese Ministry of Education, Science and Culture to Y.K., S.S., M.K., H.O. and S.U. and partly by the Okano Project of TARA, University of Tsukuba and Uehara Foundation and Proposal-Based Advanced Industrial Technology R&D Program to M.K. and S.U.. S.S. is a research fellow of the Japan Society for the Promotion of Science.",

year = "1997",

month = feb,

day = "20",

doi = "10.1016/S0378-1119(96)00673-7",

language = "English",

volume = "186",

pages = "21--27",

journal = "Gene",

issn = "0378-1119",

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number = "1",

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TY - JOUR

T1 - Structural properties and RNA-binding activities of two RNA recognition motifs of a mouse neural RNA-binding protein, mouse-Musashi-1

AU - Kurihara, Yasuyuki

AU - Nagata, Takashi

AU - Imai, Takao

AU - Hiwatashi, Ado

AU - Horiuchi, Masataka

AU - Sakakibara, Shin ichi

AU - Katahira, Masato

AU - Okano, Hideyuki

AU - Uesugi, Seiichi

N1 - Funding Information: The authors thank Mr. M. Miyauchi and M. Ohtsu for their skillful technical assistance, and Dr. M. Okabe for a kind help in preparing the manuscript. This work was supported by grants from the Japanese Ministry of Education, Science and Culture to Y.K., S.S., M.K., H.O. and S.U. and partly by the Okano Project of TARA, University of Tsukuba and Uehara Foundation and Proposal-Based Advanced Industrial Technology R&D Program to M.K. and S.U.. S.S. is a research fellow of the Japan Society for the Promotion of Science.

PY - 1997/2/20

Y1 - 1997/2/20

N2 - mouse-Musashi-1 (m-Msi-1) is an RNA-binding protein, abundantly expressed in the developing mammalian central nervous system (CNS). m-Msi-1 contains two RNA recognition motifs (RRMs). In this study, we found that the N-terminal RRM of m-Msi-1 (MMA) binds strongly to poly(G) and weakly to poly(U) in a way similar to that of the full-length m-Msi-1 protein characterized previously. The C-terminal RRM of m-Msi-1 (MMB), however, does not bind to RNA. In addition, the circular dichroism (CD) spectra of the two RRMs showed that the α-helical content of MMA is significantly higher than that of MMB, indicating that some differences in the secondary structure may be responsible for the distinct RNA binding properties of MMA and MMB.

AB - mouse-Musashi-1 (m-Msi-1) is an RNA-binding protein, abundantly expressed in the developing mammalian central nervous system (CNS). m-Msi-1 contains two RNA recognition motifs (RRMs). In this study, we found that the N-terminal RRM of m-Msi-1 (MMA) binds strongly to poly(G) and weakly to poly(U) in a way similar to that of the full-length m-Msi-1 protein characterized previously. The C-terminal RRM of m-Msi-1 (MMB), however, does not bind to RNA. In addition, the circular dichroism (CD) spectra of the two RRMs showed that the α-helical content of MMA is significantly higher than that of MMB, indicating that some differences in the secondary structure may be responsible for the distinct RNA binding properties of MMA and MMB.

KW - Circular dichroism

KW - Elav

KW - Musashi

KW - Northwestern blotting

KW - Secondary structure

KW - U1 snRNP A

KW - α-helix

KW - β-sheet

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AN - SCOPUS:0031579450

VL - 186

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JO - Gene

JF - Gene

SN - 0378-1119

IS - 1

ER -

Structural properties and RNA-binding activities of two RNA recognition motifs of a mouse neural RNA-binding protein, mouse-Musashi-1

Abstract

Keywords

ASJC Scopus subject areas

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