Structure-affinity relationship study of bleomycins and shble protein by use of a chemical array

Isao Miyazaki, Hideo Okumura, Siro Simizu, Yoshikazu Takahashi, Naoki Kanoh, Yasuhiko Muraoka, Yoshiaki Nonomura, Hiroyuki Osada

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The photocrosslinked chemical array format is useful not merely for screening protein ligands, but also for gaining insight into structure-affinity relationships (SARs). By probing an array of 2000 natural products, containing 50 bleomycin (BLM) derivatives, with cell lysates that overexpress RFP-fused Shble protein, we successfully observed interactions between Shble protein and BLMs on the array. Among the BLM derivatives, those that had long C-terminal tails were found to bind strongly. The binding signal intensities observed on the chemical array correlated well with the association constants, which were determined by isothermal titration carolimetry (ITC) experiments (r2=0.663), showing that the on-chip results were not an artifact of ligand immobilization. In addition to the C-terminal tails, the propionamide moieties in pyrimidoblamic acid (PBA) also appeared to be important for binding. The contributions of the propionamide moieties of PBA to binding were further supported by the X-ray structure of the complex of Shble protein and BLM A6. These results provide insight into the structural requirements for recognition of BLMs by Shble protein.

Original languageEnglish
Pages (from-to)845-852
Number of pages8
JournalChemBioChem
Volume10
Issue number5
DOIs
Publication statusPublished - 2009 Mar 23
Externally publishedYes

Keywords

  • Arrays
  • Bleomycins
  • Photochemistry
  • Shble protein
  • Structure-activity relationships

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

Fingerprint Dive into the research topics of 'Structure-affinity relationship study of bleomycins and shble protein by use of a chemical array'. Together they form a unique fingerprint.

  • Cite this

    Miyazaki, I., Okumura, H., Simizu, S., Takahashi, Y., Kanoh, N., Muraoka, Y., Nonomura, Y., & Osada, H. (2009). Structure-affinity relationship study of bleomycins and shble protein by use of a chemical array. ChemBioChem, 10(5), 845-852. https://doi.org/10.1002/cbic.200800728