Abstract
OBJECTIVES: Mutation in the Pkhd1 gene that encodes a ciliary protein, fibrocystin, causes multiple cysts in the kidneys and liver in the polycystic kidney (PCK) rat, a model for human autosomal recessive PCK disease. To clarify the role of primary cilia in the pancreatic duct, we examined the structure and function of the exocrine pancreas of PCK rats. METHODS: Pancreatic juice and bile were collected from anesthetized rats. Pancreatic ductal structure was analyzed by microdissection and immunohistchemistry. RESULTS: Histologically pancreatic acini were apparently normal, and no cysts were detected in the pancreas. Larger pancreatic ducts were irregularly dilated with enhanced expression of AQP1 in epithelial cells. The pancreatic duct of PCK rats exhibited significantly (P < 0.05) higher distensibility than that of wild-type (WT) rat at a physiological luminal pressure (3 cm H2O). Pancreatic fluid secretion stimulated with a physiological dose of secretin (0.03 nmol/kg per hour) in PCK rats was significantly smaller than that in WT, but the differences were not significant at higher doses. The amylase responses to carbamylcholine were not different between PCK and WT rats. CONCLUSIONS: These findings suggest that fibrocystin/primary cilia-dependent mechanisms may play a role in the regulation of pancreatic ductal structure and fluid secretion.
| Original language | English |
|---|---|
| Pages (from-to) | 1292-1298 |
| Number of pages | 7 |
| Journal | Pancreas |
| Volume | 41 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2012 Nov |
| Externally published | Yes |
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Keywords
- carbamylcholine
- fibrocystin
- pancreatic fluid secretion
- primary cilia
- secretin
ASJC Scopus subject areas
- Hepatology
- Internal Medicine
- Endocrinology
- Endocrinology, Diabetes and Metabolism
Cite this
Structure and function of the pancreas in the polycystic kidney rat. / Yi, Lanjuan; Naruse, Satoru; Furuya, Sonoko; Yamamoto, Akiko; Nakakuki, Miyuki; Nagao, Shizuko; Yoshihara, Daisuke; Ko, Shigeru; Wei, Muxin; Kondo, Takaharu; Ishiguro, Hiroshi.
In: Pancreas, Vol. 41, No. 8, 11.2012, p. 1292-1298.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Structure and function of the pancreas in the polycystic kidney rat
AU - Yi, Lanjuan
AU - Naruse, Satoru
AU - Furuya, Sonoko
AU - Yamamoto, Akiko
AU - Nakakuki, Miyuki
AU - Nagao, Shizuko
AU - Yoshihara, Daisuke
AU - Ko, Shigeru
AU - Wei, Muxin
AU - Kondo, Takaharu
AU - Ishiguro, Hiroshi
PY - 2012/11
Y1 - 2012/11
N2 - OBJECTIVES: Mutation in the Pkhd1 gene that encodes a ciliary protein, fibrocystin, causes multiple cysts in the kidneys and liver in the polycystic kidney (PCK) rat, a model for human autosomal recessive PCK disease. To clarify the role of primary cilia in the pancreatic duct, we examined the structure and function of the exocrine pancreas of PCK rats. METHODS: Pancreatic juice and bile were collected from anesthetized rats. Pancreatic ductal structure was analyzed by microdissection and immunohistchemistry. RESULTS: Histologically pancreatic acini were apparently normal, and no cysts were detected in the pancreas. Larger pancreatic ducts were irregularly dilated with enhanced expression of AQP1 in epithelial cells. The pancreatic duct of PCK rats exhibited significantly (P < 0.05) higher distensibility than that of wild-type (WT) rat at a physiological luminal pressure (3 cm H2O). Pancreatic fluid secretion stimulated with a physiological dose of secretin (0.03 nmol/kg per hour) in PCK rats was significantly smaller than that in WT, but the differences were not significant at higher doses. The amylase responses to carbamylcholine were not different between PCK and WT rats. CONCLUSIONS: These findings suggest that fibrocystin/primary cilia-dependent mechanisms may play a role in the regulation of pancreatic ductal structure and fluid secretion.
AB - OBJECTIVES: Mutation in the Pkhd1 gene that encodes a ciliary protein, fibrocystin, causes multiple cysts in the kidneys and liver in the polycystic kidney (PCK) rat, a model for human autosomal recessive PCK disease. To clarify the role of primary cilia in the pancreatic duct, we examined the structure and function of the exocrine pancreas of PCK rats. METHODS: Pancreatic juice and bile were collected from anesthetized rats. Pancreatic ductal structure was analyzed by microdissection and immunohistchemistry. RESULTS: Histologically pancreatic acini were apparently normal, and no cysts were detected in the pancreas. Larger pancreatic ducts were irregularly dilated with enhanced expression of AQP1 in epithelial cells. The pancreatic duct of PCK rats exhibited significantly (P < 0.05) higher distensibility than that of wild-type (WT) rat at a physiological luminal pressure (3 cm H2O). Pancreatic fluid secretion stimulated with a physiological dose of secretin (0.03 nmol/kg per hour) in PCK rats was significantly smaller than that in WT, but the differences were not significant at higher doses. The amylase responses to carbamylcholine were not different between PCK and WT rats. CONCLUSIONS: These findings suggest that fibrocystin/primary cilia-dependent mechanisms may play a role in the regulation of pancreatic ductal structure and fluid secretion.
KW - carbamylcholine
KW - fibrocystin
KW - pancreatic fluid secretion
KW - primary cilia
KW - secretin
UR - http://www.scopus.com/inward/record.url?scp=84868300057&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84868300057&partnerID=8YFLogxK
U2 - 10.1097/MPA.0b013e31824c12f9
DO - 10.1097/MPA.0b013e31824c12f9
M3 - Article
C2 - 22647734
AN - SCOPUS:84868300057
VL - 41
SP - 1292
EP - 1298
JO - Pancreas
JF - Pancreas
SN - 0885-3177
IS - 8
ER -