Structure assessment of regenerated cartilage using NMR spectroscopy

Shogo Miyata, Kazuhiro Homma, Katsuko Furukawa, Takashi Ushida, Tetsuya Tateishi

Research output: Contribution to journalArticle

Abstract

Recently, many kinds of methodology to regenerate articular cartilage have been developed. It is important to assess whether reconstructed cartilaginous tissue have appropriate molecular-structure for articular cartilage. There are numerous studies about biochemical and biomechanical properties of reconstructed cartilaginous tissue. However the noninvasive or nondestructive assessment of molecular-structure have not been performed on regenerated cartilage. In this study, we established the methodology of nondestructive molecular-structure assessment of regenerated cartilage using NMR spectroscopy, and performed the assessment on regenerated cartilaginous tissue prepared by chondrocyte-agarose culture model. As the results, the 1 H-NMR spectrum of the regenerated cartilaginous tissue showed peaks dominated similarly to chondroitin sulfate solution. In conclusion, this methodology using NMR spectroscopy could be useful for the assessment of regenerated cartilage.

Original languageEnglish
Pages (from-to)1237-1242
Number of pages6
JournalNihon Kikai Gakkai Ronbunshu, C Hen/Transactions of the Japan Society of Mechanical Engineers, Part C
Volume72
Issue number4
DOIs
Publication statusPublished - 2006

Keywords

  • Diagnostics
  • Human Engineering
  • Measurement
  • NMR
  • Regenerated Cartilage
  • Regenerative Medicine

ASJC Scopus subject areas

  • Mechanics of Materials
  • Mechanical Engineering
  • Industrial and Manufacturing Engineering

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