Structure of acidic polysaccharide from cell wall of Propionibacterium acnes strain C7

Masato Nagaoka, Kei Ichi Kamisango, Hideji Fujii, Kei Ichi Uchikawa, Isao Sekikawa, Ichiro Azuma

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23 Citations (Scopus)

Abstract

The structure of polysaccharide prepared by lysozyme digestion from the cell wall of Propionibacterium acnes strain C7 was examined. The polysaccharide fraction was composed of glucose, galactose, mannose, galactosamine, and diaminoman-nuronic acid in a molar ratio of 1: 1: 0.3: 1: 2. By Smith degradation of the polysaccharide, diaminouronic acid-containing fractions were obtained, and the configuration of diaminouronic acid was identified as 2,3-diacetamido-2,3-dideox-ymannuronic acid [Man(NAc)2A] by means of 1H-NMR and 13C-NMR spectro-scopic analyses. The results of analyses involving methylation and partial acid hydrolysis led to the conclusion that the polysaccharide has the repeating unit↑ 6)Gal(αl ↑4)Man(NAc)2A(β1 -6)Glc(αl ↑4) Man(NAc)2A(β1 ↑3)GalNAc(β1↑. In addition, a portion of the galactose residues were substituted at C-4 by al ↑2 linked mannotriose.

Original languageEnglish
Pages (from-to)1669-1678
Number of pages10
JournalJournal of biochemistry
Volume97
Issue number6
DOIs
Publication statusPublished - 1985 Jun
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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