TY - JOUR
T1 - Structure of acidic polysaccharide from cell wall of Propionibacterium acnes strain C7
AU - Nagaoka, Masato
AU - Kamisango, Kei Ichi
AU - Fujii, Hideji
AU - Uchikawa, Kei Ichi
AU - Sekikawa, Isao
AU - Azuma, Ichiro
PY - 1985/6
Y1 - 1985/6
N2 - The structure of polysaccharide prepared by lysozyme digestion from the cell wall of Propionibacterium acnes strain C7 was examined. The polysaccharide fraction was composed of glucose, galactose, mannose, galactosamine, and diaminoman-nuronic acid in a molar ratio of 1: 1: 0.3: 1: 2. By Smith degradation of the polysaccharide, diaminouronic acid-containing fractions were obtained, and the configuration of diaminouronic acid was identified as 2,3-diacetamido-2,3-dideox-ymannuronic acid [Man(NAc)2A] by means of 1H-NMR and 13C-NMR spectro-scopic analyses. The results of analyses involving methylation and partial acid hydrolysis led to the conclusion that the polysaccharide has the repeating unit↑ 6)Gal(αl ↑4)Man(NAc)2A(β1 -6)Glc(αl ↑4) Man(NAc)2A(β1 ↑3)GalNAc(β1↑. In addition, a portion of the galactose residues were substituted at C-4 by al ↑2 linked mannotriose.
AB - The structure of polysaccharide prepared by lysozyme digestion from the cell wall of Propionibacterium acnes strain C7 was examined. The polysaccharide fraction was composed of glucose, galactose, mannose, galactosamine, and diaminoman-nuronic acid in a molar ratio of 1: 1: 0.3: 1: 2. By Smith degradation of the polysaccharide, diaminouronic acid-containing fractions were obtained, and the configuration of diaminouronic acid was identified as 2,3-diacetamido-2,3-dideox-ymannuronic acid [Man(NAc)2A] by means of 1H-NMR and 13C-NMR spectro-scopic analyses. The results of analyses involving methylation and partial acid hydrolysis led to the conclusion that the polysaccharide has the repeating unit↑ 6)Gal(αl ↑4)Man(NAc)2A(β1 -6)Glc(αl ↑4) Man(NAc)2A(β1 ↑3)GalNAc(β1↑. In addition, a portion of the galactose residues were substituted at C-4 by al ↑2 linked mannotriose.
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U2 - 10.1093/oxfordjournals.jbchem.a135225
DO - 10.1093/oxfordjournals.jbchem.a135225
M3 - Article
C2 - 4030744
AN - SCOPUS:0021866342
SN - 0021-924X
VL - 97
SP - 1669
EP - 1678
JO - Journal of Biochemistry
JF - Journal of Biochemistry
IS - 6
ER -