TY - JOUR
T1 - Structure of the dnaA region of Pseudomonas putida
T2 - Conservation among three bacteria, Bacillus subtilis, Escherichia coli and P. putida
AU - Fujita, Masaki Q.
AU - Yoshikawa, Hiroshi
AU - Ogasawara, Naotake
PY - 1989/2
Y1 - 1989/2
N2 - We have cloned from Pseudomonas putida a gene homologous to Escherichia coli dnaA, and determined the sequence of the gene and its neighboring region. The dnaA gene and at least three other genes, dnaN, recF and gyrB, were found to be highly homologous to the genes in the dnaA regions of the E. coli and Bacillus subtilis chromosomes. A non-translatable region of some 600 bp immediately upstream of the dnaA gene is also conserved in the three bacteria and contains 3, 12, and 14 DnaA-boxes (TTATCCACA and closely related sequences) in E. coli, P. putida and B. subtilis, respectively. The present results confirm our hypothesis that the dnaA region is the replication origin region of the ancestral bacterium and that the essential feature of the dnaA and DnaA-box combination is conserved in most eubacteria and plays a central role in initiation of chromosomal replication.
AB - We have cloned from Pseudomonas putida a gene homologous to Escherichia coli dnaA, and determined the sequence of the gene and its neighboring region. The dnaA gene and at least three other genes, dnaN, recF and gyrB, were found to be highly homologous to the genes in the dnaA regions of the E. coli and Bacillus subtilis chromosomes. A non-translatable region of some 600 bp immediately upstream of the dnaA gene is also conserved in the three bacteria and contains 3, 12, and 14 DnaA-boxes (TTATCCACA and closely related sequences) in E. coli, P. putida and B. subtilis, respectively. The present results confirm our hypothesis that the dnaA region is the replication origin region of the ancestral bacterium and that the essential feature of the dnaA and DnaA-box combination is conserved in most eubacteria and plays a central role in initiation of chromosomal replication.
KW - Conservation in eubacteria
KW - DnaA protein
KW - DnaA-box
KW - dnaA region
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U2 - 10.1007/BF00427033
DO - 10.1007/BF00427033
M3 - Article
C2 - 2540413
AN - SCOPUS:0024615726
SN - 1617-4615
VL - 215
SP - 381
EP - 387
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
IS - 3
ER -