Structures and intracellular delivery of pDNA/sugar-modified chitosan complexes

Toshinori Sato, Mayu Hashimoto, Minoru Morimoto, Hiroyuki Saimoto, Yoshihiro Shigemasa, Hironobu Yanagie

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Chitosan is a useful nonviral vector for gene delivery. In order to make a pDNA/chitosan complex specific to hepatocytes and macrophages, lactose-or mannose-modified chitosan (lac-chitosan or man-chitosan) was synthesized. 8%lac-chitosan showed excellent DNA-binding ability, good protection of DNA from nuclease, and the suppression of self-aggregation and serum-induced aggregation. The cell transfection efficiency of the pDNA/8%lac-chitosan complexes was greater for HepG2 cells. Observations with a confocal laser scanning microscope indicated that the pDNA/lac-chitosan complexes were delivered to the late endosome and have the advantage of delivering DNA to the perinuclear region. The cellular uptake of pDNA/man-chitosan complexes to mause peritoneal macrophages was observed. The pDNA/man-chitosan complexes showed much higher transfection efficiency than pDNA/chitosan complexes. Observation with a confocal laser microscope suggested differences in the cellular uptake mechanism between pDNA/chitosan complexes and pDNA/man-chitosan complexes.

Original languageEnglish
Title of host publicationPolymer Preprints, Japan
Pages5371-5372
Number of pages2
Volume55
Edition2
Publication statusPublished - 2006
Event55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan
Duration: 2006 Sep 202006 Sep 22

Other

Other55th Society of Polymer Science Japan Symposium on Macromolecules
CountryJapan
CityToyama
Period06/9/2006/9/22

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Keywords

  • Carbohydrate recognition
  • Chitosan
  • Gene transfer
  • Macrophage, hapatocyte
  • Plasmid DNA

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Sato, T., Hashimoto, M., Morimoto, M., Saimoto, H., Shigemasa, Y., & Yanagie, H. (2006). Structures and intracellular delivery of pDNA/sugar-modified chitosan complexes. In Polymer Preprints, Japan (2 ed., Vol. 55, pp. 5371-5372)