Structures, synthesis, and human nod1 stimulation of immunostimulatory bacterial peptidoglycan fragments in the environment

Yukari Fujimoto, Koichi Fukase

Research output: Contribution to journalReview article

14 Citations (Scopus)

Abstract

Bacteria release immunostimulatory compounds to the environment, and one of the stimulants is the ligand of nucleotide-binding oligomerization domain protein 1 (Nod1), an intracellular protein involved in the recognition of the bacterial component peptidoglycans having a diaminopimelic acid (DAP) structure. The polymorphisms of Nod1 have been linked to several inflammatory diseases and allergies that are strongly affected by environmental factors. The present paper summarizes recent results on the isolation and structural elucidation of natural human Nod1 (hNod1) ligands from the Escherichia coli (E. coli) K-12 culture supernatant, the first chemical synthesis of these natural ligands and related PGN fragments structures, and the hNod1 stimulatory activities of the chemically synthesized DAP-type PGN fragments. For structural characterization studies, the 7-(diethylamino)coumarin-3-carbonyl (DEAC) labeling method was also used to enhance the sensitivity in mass spectrometry studies, in order to observe PGN fragments in a comprehensive manner. The results suggest that DAP-containing bacteria release certain hNod1 ligands to the environment and that these ligands accumulate in the environment and regulate the immune system through Nod1.

Original languageEnglish
Pages (from-to)518-525
Number of pages8
JournalJournal of Natural Products
Volume74
Issue number3
DOIs
Publication statusPublished - 2011 Mar 25
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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