Studies of a microchip flow-chamber system to characterize whole blood thrombogenicity in healthy individuals

Yusuke Yamaguchi, Takanori Moriki, Atsuko Igari, Yumiko Matsubara, Tomoko Ohnishi, Kazuya Hosokawa, Mitsuru Murata

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Introduction A whole blood flow-chamber system, the Total Thrombus-formation Analysis System (T-TAS), was developed for quantitative analysis of platelet thrombus formation (PTF) using microchips with thrombogenic surfaces (collagen, PL chip; collagen plus tissue thromboplastin, AR chip) under shear stress conditions. We evaluated the usefulness of the T-TAS for assessing individual thrombogenicity compared with other platelet function tests. Materials and Methods Blood samples from 31 healthy volunteers were applied to the T-TAS to measure PTF starting time (T10: time to reach 10 kPa), occlusion time (T60 for PL chip; T80 for AR chip), and area under the curve (AUC10, area under curve until10 min for PL chip; AUC30, 30 min for AR chip) under various shear rates (1000, 1500, 2000 s- 1 for PL chip; 300 s- 1 for AR chip). Platelet functions were also tested using platelet aggregometry, the PFA-100 (collagen and epinephrine [C/EPI], collagen and adenosine diphosphate [C/ADP]), and the VerifyNow P2Y12 assay. Results Individual pressure waveforms, including PTF starting and ending points, varied among healthy subjects. In the PL chip, T10 and AUC10 showed a shear-dependent correlation with C/EPI or C/ADP. VerifyNow P2Y12 values were not significantly associated with the parameters of the T-TAS. Platelet counts were correlated with all AR measurements, and mostly with PL measurements. Conclusion The results of the T-TAS were associated with those of the PFA-100 in many respects, indicating that its characteristics are related to shear-induced PTF. The T-TAS showed few correlations with platelet aggregometry and the VerifyNow P2Y12 assay. The T-TAS may allow for the measurement of comprehensive parameters of individual thrombogenicity under whole blood flow conditions.

Original languageEnglish
Pages (from-to)263-270
Number of pages8
JournalThrombosis Research
Volume132
Issue number2
DOIs
Publication statusPublished - 2013 Aug

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Thrombosis
Blood Platelets
Collagen
Adenosine Diphosphate
Epinephrine
Area Under Curve
Healthy Volunteers
Platelet Function Tests
Thromboplastin
Platelet Count
Pressure

Keywords

  • Flow-chamber system
  • Platelet
  • Thrombogenicity
  • Thrombus formation

ASJC Scopus subject areas

  • Hematology

Cite this

Studies of a microchip flow-chamber system to characterize whole blood thrombogenicity in healthy individuals. / Yamaguchi, Yusuke; Moriki, Takanori; Igari, Atsuko; Matsubara, Yumiko; Ohnishi, Tomoko; Hosokawa, Kazuya; Murata, Mitsuru.

In: Thrombosis Research, Vol. 132, No. 2, 08.2013, p. 263-270.

Research output: Contribution to journalArticle

Yamaguchi, Yusuke ; Moriki, Takanori ; Igari, Atsuko ; Matsubara, Yumiko ; Ohnishi, Tomoko ; Hosokawa, Kazuya ; Murata, Mitsuru. / Studies of a microchip flow-chamber system to characterize whole blood thrombogenicity in healthy individuals. In: Thrombosis Research. 2013 ; Vol. 132, No. 2. pp. 263-270.
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AU - Yamaguchi, Yusuke

AU - Moriki, Takanori

AU - Igari, Atsuko

AU - Matsubara, Yumiko

AU - Ohnishi, Tomoko

AU - Hosokawa, Kazuya

AU - Murata, Mitsuru

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N2 - Introduction A whole blood flow-chamber system, the Total Thrombus-formation Analysis System (T-TAS), was developed for quantitative analysis of platelet thrombus formation (PTF) using microchips with thrombogenic surfaces (collagen, PL chip; collagen plus tissue thromboplastin, AR chip) under shear stress conditions. We evaluated the usefulness of the T-TAS for assessing individual thrombogenicity compared with other platelet function tests. Materials and Methods Blood samples from 31 healthy volunteers were applied to the T-TAS to measure PTF starting time (T10: time to reach 10 kPa), occlusion time (T60 for PL chip; T80 for AR chip), and area under the curve (AUC10, area under curve until10 min for PL chip; AUC30, 30 min for AR chip) under various shear rates (1000, 1500, 2000 s- 1 for PL chip; 300 s- 1 for AR chip). Platelet functions were also tested using platelet aggregometry, the PFA-100 (collagen and epinephrine [C/EPI], collagen and adenosine diphosphate [C/ADP]), and the VerifyNow P2Y12 assay. Results Individual pressure waveforms, including PTF starting and ending points, varied among healthy subjects. In the PL chip, T10 and AUC10 showed a shear-dependent correlation with C/EPI or C/ADP. VerifyNow P2Y12 values were not significantly associated with the parameters of the T-TAS. Platelet counts were correlated with all AR measurements, and mostly with PL measurements. Conclusion The results of the T-TAS were associated with those of the PFA-100 in many respects, indicating that its characteristics are related to shear-induced PTF. The T-TAS showed few correlations with platelet aggregometry and the VerifyNow P2Y12 assay. The T-TAS may allow for the measurement of comprehensive parameters of individual thrombogenicity under whole blood flow conditions.

AB - Introduction A whole blood flow-chamber system, the Total Thrombus-formation Analysis System (T-TAS), was developed for quantitative analysis of platelet thrombus formation (PTF) using microchips with thrombogenic surfaces (collagen, PL chip; collagen plus tissue thromboplastin, AR chip) under shear stress conditions. We evaluated the usefulness of the T-TAS for assessing individual thrombogenicity compared with other platelet function tests. Materials and Methods Blood samples from 31 healthy volunteers were applied to the T-TAS to measure PTF starting time (T10: time to reach 10 kPa), occlusion time (T60 for PL chip; T80 for AR chip), and area under the curve (AUC10, area under curve until10 min for PL chip; AUC30, 30 min for AR chip) under various shear rates (1000, 1500, 2000 s- 1 for PL chip; 300 s- 1 for AR chip). Platelet functions were also tested using platelet aggregometry, the PFA-100 (collagen and epinephrine [C/EPI], collagen and adenosine diphosphate [C/ADP]), and the VerifyNow P2Y12 assay. Results Individual pressure waveforms, including PTF starting and ending points, varied among healthy subjects. In the PL chip, T10 and AUC10 showed a shear-dependent correlation with C/EPI or C/ADP. VerifyNow P2Y12 values were not significantly associated with the parameters of the T-TAS. Platelet counts were correlated with all AR measurements, and mostly with PL measurements. Conclusion The results of the T-TAS were associated with those of the PFA-100 in many respects, indicating that its characteristics are related to shear-induced PTF. The T-TAS showed few correlations with platelet aggregometry and the VerifyNow P2Y12 assay. The T-TAS may allow for the measurement of comprehensive parameters of individual thrombogenicity under whole blood flow conditions.

KW - Flow-chamber system

KW - Platelet

KW - Thrombogenicity

KW - Thrombus formation

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