Studies of assisted reproduction techniques (ART) for HIV-1-discordant couples using washed sperm and the nested PCR method: A comparison of the pregnancy rates in HIV-1-discordant couples and control couples

Katsunori Kashima, Koichi Takakuwa, Mina Suzuki, Mayuko Makino, Satoru Kaneko, Shingo Kato, Hideji Hanabusa, Kenichi Tanaka

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In this study, the efficacy and safety of assisted reproduction techniques with the sperm-washing method and nested PCR assay were evaluated in HIV-1-discordant couples, as many HIV-1-positive people of reproductive age are getting married and wish to have children safely. Twenty-seven HIV-1-discordant couples (husband, positive; wife, negative) were enrolled in this study. The spermatozoa were separated from semen samples by density gradient centrifugation and the swim-up method. HIV-1 RNA and proviral DNA were checked using nested PCR with a detection limit of one copy before fertilization and before embryo transfer. Clinical outcomes were compared with those of matched control couples. Thirty-eight cycles of in vitro fertilization or intracytoplasmic sperm injection were performed in HIV-1-discordant couples, where the pregnancy rates per embryo transfer and per couple were 60.6 and 63.0%, respectively. These rates were significantly higher than those in control couples (P < 0.05). Furthermore, all of the females and babies remained HIV-1 negative throughout the study period. Our data strongly suggest that this technique will allow HIV-1-discordant couples to conceive more safely and effectively.

Original languageEnglish
Pages (from-to)173-176
Number of pages4
JournalJapanese Journal of Infectious Diseases
Issue number3
Publication statusPublished - 2009 Sep 2


ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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