Study for the Ho: YAG laser - intervertebral disc cell interaction using three-dimensional cell culture system

Masato Sato, Miya Ishihara, Tsunenori Arai, Takashi Asazuma, Toshiyuki Kikuchi, Makoto Kikuchi, Kyosuke Fujikawa

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

The purpose of this study is to evaluate the influence on the intervertebral disc cells after laser irradiation using three- dimensional culture system and to clarify the optimum Ho:YAG laser irradiation condition on percutaneous laser disc decompression (PLDD) for lumbar disc herniation. Since the Ho:YAG laser ablation is characterized by water-vapor bubble dynamics, not only thermal effect but also acoustic effect on cell metabolism might occur in the intervertebral disc. We studied the disc cell reaction from the metabolic point of view to investigate photothermal and photoacoustic effects on three-dimensional cultured disc cell. Intervertebral discs were obtained from female 30 Japanese white rabbits weighing about 1 kg. A pulsed Ho:YAG laser (wavelength: 2.1 μm, pulse width: about 200μs) was delivered through a 200μm-core diameter single silica glass fiber. We used the Ho:YAG laser irradiation fluence ranging from 60 - 800 J/cm2 at the fiber end. To investigate acoustic effect, the acoustic transducer constructed with polyvinylidene fluoride (PVdF) film and acoustic absorber was used to detect the stress wave. Thermocouple and thermography were used to investigate thermal effect. Concerning damage of plasma membrane and ability of matrix synthesis, thermal effect might mainly affect cell reaction in total energy of 54J (closed to practically used condition), but in 27J, acoustic effect might contribute to it. We found that total energy was key parameter among the optimum condition, so that temperature and/or stress wave may influence Ho:YAG laser - disc cell interactions.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherSociety of Photo-Optical Instrumentation Engineers
Pages216-221
Number of pages6
Volume3914
Publication statusPublished - 2000
Externally publishedYes
EventLaser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical - San Jose, CA, USA
Duration: 2000 Jan 222000 Jan 27

Other

OtherLaser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical
CitySan Jose, CA, USA
Period00/1/2200/1/27

Fingerprint

intervertebral disks
Videodisks
Cell culture
YAG lasers
Laser beam effects
Acoustics
Thermal effects
cells
acoustics
temperature effects
stress waves
interactions
Acoustic transducers
irradiation
Photoacoustic effect
Weighing
Laser ablation
Cell membranes
Fused silica
Thermocouples

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Sato, M., Ishihara, M., Arai, T., Asazuma, T., Kikuchi, T., Kikuchi, M., & Fujikawa, K. (2000). Study for the Ho: YAG laser - intervertebral disc cell interaction using three-dimensional cell culture system. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 3914, pp. 216-221). Society of Photo-Optical Instrumentation Engineers.

Study for the Ho : YAG laser - intervertebral disc cell interaction using three-dimensional cell culture system. / Sato, Masato; Ishihara, Miya; Arai, Tsunenori; Asazuma, Takashi; Kikuchi, Toshiyuki; Kikuchi, Makoto; Fujikawa, Kyosuke.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3914 Society of Photo-Optical Instrumentation Engineers, 2000. p. 216-221.

Research output: Chapter in Book/Report/Conference proceedingChapter

Sato, M, Ishihara, M, Arai, T, Asazuma, T, Kikuchi, T, Kikuchi, M & Fujikawa, K 2000, Study for the Ho: YAG laser - intervertebral disc cell interaction using three-dimensional cell culture system. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 3914, Society of Photo-Optical Instrumentation Engineers, pp. 216-221, Laser-Tissue Interaction XI: Photochemical, Photothermal, and Photomechanical, San Jose, CA, USA, 00/1/22.
Sato M, Ishihara M, Arai T, Asazuma T, Kikuchi T, Kikuchi M et al. Study for the Ho: YAG laser - intervertebral disc cell interaction using three-dimensional cell culture system. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3914. Society of Photo-Optical Instrumentation Engineers. 2000. p. 216-221
Sato, Masato ; Ishihara, Miya ; Arai, Tsunenori ; Asazuma, Takashi ; Kikuchi, Toshiyuki ; Kikuchi, Makoto ; Fujikawa, Kyosuke. / Study for the Ho : YAG laser - intervertebral disc cell interaction using three-dimensional cell culture system. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 3914 Society of Photo-Optical Instrumentation Engineers, 2000. pp. 216-221
@inbook{bd4d256042394a6fb9b14aee7a715423,
title = "Study for the Ho: YAG laser - intervertebral disc cell interaction using three-dimensional cell culture system",
abstract = "The purpose of this study is to evaluate the influence on the intervertebral disc cells after laser irradiation using three- dimensional culture system and to clarify the optimum Ho:YAG laser irradiation condition on percutaneous laser disc decompression (PLDD) for lumbar disc herniation. Since the Ho:YAG laser ablation is characterized by water-vapor bubble dynamics, not only thermal effect but also acoustic effect on cell metabolism might occur in the intervertebral disc. We studied the disc cell reaction from the metabolic point of view to investigate photothermal and photoacoustic effects on three-dimensional cultured disc cell. Intervertebral discs were obtained from female 30 Japanese white rabbits weighing about 1 kg. A pulsed Ho:YAG laser (wavelength: 2.1 μm, pulse width: about 200μs) was delivered through a 200μm-core diameter single silica glass fiber. We used the Ho:YAG laser irradiation fluence ranging from 60 - 800 J/cm2 at the fiber end. To investigate acoustic effect, the acoustic transducer constructed with polyvinylidene fluoride (PVdF) film and acoustic absorber was used to detect the stress wave. Thermocouple and thermography were used to investigate thermal effect. Concerning damage of plasma membrane and ability of matrix synthesis, thermal effect might mainly affect cell reaction in total energy of 54J (closed to practically used condition), but in 27J, acoustic effect might contribute to it. We found that total energy was key parameter among the optimum condition, so that temperature and/or stress wave may influence Ho:YAG laser - disc cell interactions.",
author = "Masato Sato and Miya Ishihara and Tsunenori Arai and Takashi Asazuma and Toshiyuki Kikuchi and Makoto Kikuchi and Kyosuke Fujikawa",
year = "2000",
language = "English",
volume = "3914",
pages = "216--221",
booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",
publisher = "Society of Photo-Optical Instrumentation Engineers",

}

TY - CHAP

T1 - Study for the Ho

T2 - YAG laser - intervertebral disc cell interaction using three-dimensional cell culture system

AU - Sato, Masato

AU - Ishihara, Miya

AU - Arai, Tsunenori

AU - Asazuma, Takashi

AU - Kikuchi, Toshiyuki

AU - Kikuchi, Makoto

AU - Fujikawa, Kyosuke

PY - 2000

Y1 - 2000

N2 - The purpose of this study is to evaluate the influence on the intervertebral disc cells after laser irradiation using three- dimensional culture system and to clarify the optimum Ho:YAG laser irradiation condition on percutaneous laser disc decompression (PLDD) for lumbar disc herniation. Since the Ho:YAG laser ablation is characterized by water-vapor bubble dynamics, not only thermal effect but also acoustic effect on cell metabolism might occur in the intervertebral disc. We studied the disc cell reaction from the metabolic point of view to investigate photothermal and photoacoustic effects on three-dimensional cultured disc cell. Intervertebral discs were obtained from female 30 Japanese white rabbits weighing about 1 kg. A pulsed Ho:YAG laser (wavelength: 2.1 μm, pulse width: about 200μs) was delivered through a 200μm-core diameter single silica glass fiber. We used the Ho:YAG laser irradiation fluence ranging from 60 - 800 J/cm2 at the fiber end. To investigate acoustic effect, the acoustic transducer constructed with polyvinylidene fluoride (PVdF) film and acoustic absorber was used to detect the stress wave. Thermocouple and thermography were used to investigate thermal effect. Concerning damage of plasma membrane and ability of matrix synthesis, thermal effect might mainly affect cell reaction in total energy of 54J (closed to practically used condition), but in 27J, acoustic effect might contribute to it. We found that total energy was key parameter among the optimum condition, so that temperature and/or stress wave may influence Ho:YAG laser - disc cell interactions.

AB - The purpose of this study is to evaluate the influence on the intervertebral disc cells after laser irradiation using three- dimensional culture system and to clarify the optimum Ho:YAG laser irradiation condition on percutaneous laser disc decompression (PLDD) for lumbar disc herniation. Since the Ho:YAG laser ablation is characterized by water-vapor bubble dynamics, not only thermal effect but also acoustic effect on cell metabolism might occur in the intervertebral disc. We studied the disc cell reaction from the metabolic point of view to investigate photothermal and photoacoustic effects on three-dimensional cultured disc cell. Intervertebral discs were obtained from female 30 Japanese white rabbits weighing about 1 kg. A pulsed Ho:YAG laser (wavelength: 2.1 μm, pulse width: about 200μs) was delivered through a 200μm-core diameter single silica glass fiber. We used the Ho:YAG laser irradiation fluence ranging from 60 - 800 J/cm2 at the fiber end. To investigate acoustic effect, the acoustic transducer constructed with polyvinylidene fluoride (PVdF) film and acoustic absorber was used to detect the stress wave. Thermocouple and thermography were used to investigate thermal effect. Concerning damage of plasma membrane and ability of matrix synthesis, thermal effect might mainly affect cell reaction in total energy of 54J (closed to practically used condition), but in 27J, acoustic effect might contribute to it. We found that total energy was key parameter among the optimum condition, so that temperature and/or stress wave may influence Ho:YAG laser - disc cell interactions.

UR - http://www.scopus.com/inward/record.url?scp=0033707519&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033707519&partnerID=8YFLogxK

M3 - Chapter

AN - SCOPUS:0033707519

VL - 3914

SP - 216

EP - 221

BT - Proceedings of SPIE - The International Society for Optical Engineering

PB - Society of Photo-Optical Instrumentation Engineers

ER -