Study of blood charring precursor states using backscattering at 663 nm from blood and optical window boundary

Mei Takahashi, Arisa Ito, Shunichiro Miyoshi, Takehiro Kimura, Seiji Takatsuki, Kotaro Fukumoto, Keiichi Fukuda, Tsunenori Arai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background and Objective Contact laser irradiation is generally used in therapeutic laser procedures such as plastic surgery and laser catheter lead removal. However, it may induce blood charring on the surface of the optical window in blood circumstance so that the laser beam might be blocked. Various charring detection methods have been proposed, but they detect charring only after charring has occurred. This study investigates the transient behavior of red blood cells (RBCs) prior to the charring on the surface of an optical window during red laser irradiation in blood circumstance. Materials and Methods The backscattering light power was continuously measured to investigate the transient behavior of a 1-mm-thick porcine blood model (hematocrit: 40%) during continuous laser irradiation (center wavelength: 663 nm; irradiance: 81 W/cm2). A rabbit blood model was microscopically observed after irradiation. The absorption coefficient (μa) and the reduced scattering coefficient (μs) were measured using a double integrating sphere setup and the inverse adding-doubling method. The backscattering light power was continuously measured in vivo during contact laser irradiation via a laser catheter in a porcine heart cavity. Results The results reveal that it may be possible to detect a precursory state of charring from a time course of the backscattering light power. μa increased monotonically by 15% until charring occurred. μs decreased by 10% followed a broad peak until charring occurred. These changes in the optical property correspond to changes in the morphology of RBCs. Changes in the backscattering light power measured in vivo were similar to those measured ex vivo. Conclusions The transient optical changes in blood prior to charring may be caused by changes in the morphology of RBCs on the optical window surface. Backscattering light power measurements may be a practical method to detect the precursor state of charring.

Original languageEnglish
Pages (from-to)508-513
Number of pages6
JournalLasers in Surgery and Medicine
Volume44
Issue number6
DOIs
Publication statusPublished - 2012 Aug 1

Keywords

  • laser catheter
  • optical properties
  • red blood cell morphology

ASJC Scopus subject areas

  • Surgery
  • Dermatology

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