Study of photosensitization reaction progress in a 96 well plate with photosensitizer rich condition using Talaporfin sodium

Emiyu Ogawa, Mei Takahashi, Tsunenori Arai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

To quantitatively investigate photosensitization reaction in vitro against myocardial cells with photosensitizer rich condition in solution using Talaporfin sodium in the well of a 96 well plate, we studied photosensitization reaction progress in this well. We have proposed non-thermal conduction block of myocardium tissue using the photosensitization reaction with laser irradiation shortly after Talaporfin sodium injection. In above situation, the photosensitizer is located outside the myocardial cells in high concentration. To understand interaction of the photosensitization reaction in which the photosensitizer distributes outside cells, the photosensitization reaction progress in the well was studied. Talaporfin sodium (799.69 MW) solution and a 663 nm diode laser were used. The photosensitizer solution concentrations of 12.5-37.5 μM were employed. The photosensitizer fluorescence with 0.29 W/cm2 in irradiance, which was optimized in previous cell death study, was measured during the laser irradiation until 40 J/cm2. The photosensitizer solution absorbance and dissolved oxygen pressure after the laser irradiation were also measured. We found that the photosensitization reaction progress had 2 distinctive phases of different reaction rate: rapid photosensitization reaction consuming dissolved oxygen and gentle photosensitization reaction with oxygen diffusion from the solution-air boundary. The dissolved oxygen pressure and photosensitizer solution absorbance were 30% and 80% of the initial values after the laser irradiation, respectively. Therefore, oxygen was rate-controlling factor of the photosensitization reaction in the well with the photosensitizer rich condition. In the oxygen diffusion phase, the oxygen pressure was maintained around 40 mmHg until the laser irradiation of 40 J/cm2 and it is similar to that of myocardium tissue in vivo. We think that our 96 well plate in vitro system may simulate PDT in myocardial tissue with photosensitization reaction parameters mentioned above.

Original languageEnglish
Title of host publicationOptical Interactions with Tissue and Cells XXIV
DOIs
Publication statusPublished - 2013 Apr 12
EventOptical Interactions with Tissue and Cells XXIV - San Francisco, CA, United States
Duration: 2013 Feb 42013 Feb 5

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8579
ISSN (Print)1605-7422

Other

OtherOptical Interactions with Tissue and Cells XXIV
CountryUnited States
CitySan Francisco, CA
Period13/2/413/2/5

Keywords

  • NPe6
  • fluorescence
  • myocardial cell
  • oxygen pressure
  • photosensitizer absorbance

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Ogawa, E., Takahashi, M., & Arai, T. (2013). Study of photosensitization reaction progress in a 96 well plate with photosensitizer rich condition using Talaporfin sodium. In Optical Interactions with Tissue and Cells XXIV [85790D] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8579). https://doi.org/10.1117/12.2003288