Study on the Allelopathy of Alfalfa (Medicago sativa L.): II. Isolation and identification of allelopathic substances in alfalfa

Kana Nakahisa, Eiji Tsuzuki, Hiroyuki Terao, Seiji Kosemura

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Allelopathic substances in alfalfa plants (Medicago sativa L.) were purified from methanol extracts of alfalfa shoots. The methanol extracts were fractionated into an acidic fraction, and the sample was divided into 10 fractions by thinlayer chromatography (TLC). Growth of radish seedlings was inhibited by the substances obtained from Rf values of 0.5–0.6 and 0.6–0.7. These fractions were eluted with distilled water, and the eluates were analyzed by high-performance liquid chromatography (HPLC). Six peaks were detected on the chromatogram. Growth of radish seedlings was inhibited on three peaks. Of these peaks, two were identified using HPLC, a mass-spectrometer, IR, and 1NMR as follows: peak b, ferulic acid and peak f, salicylic acid. The growth of alfalfa and radish seedlings was inhibited at lower concentrations of these substances.

Original languageEnglish
Pages (from-to)278-284
Number of pages7
JournalJapanese Journal of Crop Science
Volume63
Issue number2
DOIs
Publication statusPublished - 1994 Jan 1

Keywords

  • Alfalfa
  • Allelopathy
  • Ferulic acid
  • Inhibitory substances
  • Injury by continuous cropping
  • Salicylic acid

ASJC Scopus subject areas

  • Food Science
  • Agronomy and Crop Science
  • Genetics

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