Study on the nature of starfish larval muscle cells in vitro

Hiroyuki Kaneko, Yukio Kawahara, Mitsumasa Okamoto, Marina Dan-Sohkawa

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We describe a culture method in which larval muscle cells of the starfish Asterias amurensis develop from epithelial cells, probably deriving from the coelomic pouches. The nature of the muscle which appears in the culture is described morphologically, physiologically and ultrastructurally. Cells were dissociated from the late gastrula stage, treated with 0.6 M of glycine in half-strength sea water free of Ca2+ and Mg2+ for 12 hr, and cultured for various periods. Elongated cells appeared after about a week from small aggregates of epithelial cells which were found among the mesenchymal network on the 1st day of culture. The characteristics of the elongated cells are as follows: (1) they possess two or more arms; (2) they adhere to the cultural substratum, mesenchyme cells, and themselves at all parts of the cell body; (3) they contract in response to acetylcholine; (4) they contain an abundance of fibrous actin and myosin throughout their cytoplasm; (5) the cytoplasm contains bundles of thick (12-19 nm) and thin (5-8 nm) filaments without any dense material; and (6) no proliferative activity was observed while the cells were kept in culture for up to 14 days. These features were compared with those of the larval muscle cells in vivo.

Original languageEnglish
Pages (from-to)287-296
Number of pages10
JournalZoological Science
Volume14
Issue number2
Publication statusPublished - 1997 Apr
Externally publishedYes

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Asteroidea
myocytes
cells
epithelial cells
cytoplasm
Asterias
acetylcholine
pouches
cell aggregates
myosin
glycine (amino acid)
actin
seawater
calcium
muscles

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Kaneko, H., Kawahara, Y., Okamoto, M., & Dan-Sohkawa, M. (1997). Study on the nature of starfish larval muscle cells in vitro. Zoological Science, 14(2), 287-296.

Study on the nature of starfish larval muscle cells in vitro. / Kaneko, Hiroyuki; Kawahara, Yukio; Okamoto, Mitsumasa; Dan-Sohkawa, Marina.

In: Zoological Science, Vol. 14, No. 2, 04.1997, p. 287-296.

Research output: Contribution to journalArticle

Kaneko, H, Kawahara, Y, Okamoto, M & Dan-Sohkawa, M 1997, 'Study on the nature of starfish larval muscle cells in vitro', Zoological Science, vol. 14, no. 2, pp. 287-296.
Kaneko H, Kawahara Y, Okamoto M, Dan-Sohkawa M. Study on the nature of starfish larval muscle cells in vitro. Zoological Science. 1997 Apr;14(2):287-296.
Kaneko, Hiroyuki ; Kawahara, Yukio ; Okamoto, Mitsumasa ; Dan-Sohkawa, Marina. / Study on the nature of starfish larval muscle cells in vitro. In: Zoological Science. 1997 ; Vol. 14, No. 2. pp. 287-296.
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