Subcellular calcium dynamics during juvenile development in mouse hippocampal astrocytes

Ryota Nakayama, Takuya Sasaki, Kenji Tanaka, Yuji Ikegaya

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Astrocytes generate calcium signals throughout their fine processes, which are assumed to locally regulate neighbouring neurotransmission and blood flow. The intercellular morphological relationships mature during juvenile periods when astrocytes elongate highly ramified processes. In this study, we examined developmental changes in calcium activity patterns of single hippocampal astrocytes using a transgenic mouse line in which astrocytes selectively express a genetically encoded calcium indicator, Yellow Cameleon-Nano50. Compared with postnatal day 7, astrocytes at postnatal day 30 showed larger subcellular calcium events and a greater proportion of somatic events. At both ages, the calcium activity was abolished by removal of extracellular calcium ions. Calcium events in late juvenile astrocytes were not affected by spontaneously occurring sharp waves that trigger synchronized neuronal spikes, implying the independence of astrocyte calcium signals from neuronal synchronization. These results demonstrate that astrocytes undergo dynamic changes in their activity patterns during juvenile development.

Original languageEnglish
Pages (from-to)923-932
Number of pages10
JournalEuropean Journal of Neuroscience
Volume43
Issue number7
DOIs
Publication statusPublished - 2016 Apr 1

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Astrocytes
Calcium
Synaptic Transmission
Transgenic Mice
Ions

Keywords

  • Astrocyte
  • Calcium
  • Hippocampus
  • Imaging

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Subcellular calcium dynamics during juvenile development in mouse hippocampal astrocytes. / Nakayama, Ryota; Sasaki, Takuya; Tanaka, Kenji; Ikegaya, Yuji.

In: European Journal of Neuroscience, Vol. 43, No. 7, 01.04.2016, p. 923-932.

Research output: Contribution to journalArticle

Nakayama, Ryota ; Sasaki, Takuya ; Tanaka, Kenji ; Ikegaya, Yuji. / Subcellular calcium dynamics during juvenile development in mouse hippocampal astrocytes. In: European Journal of Neuroscience. 2016 ; Vol. 43, No. 7. pp. 923-932.
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