Subcellular Imbalances in Synaptic Activity

Naoya Takahashi, Chiaki Kobayashi, Tomoe Ishikawa, Yuji Ikegaya

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The dynamic interactions between synaptic excitation and inhibition (E/I) shape membrane potential fluctuations and determine patterns of neuronal outputs; however, the spatiotemporal organization of these interactions within a single cell is poorly understood. Here, we investigated the relationship between local synaptic excitation and global inhibition in hippocampal pyramidal neurons using functional dendrite imaging in combination with whole-cell recordings of inhibitory postsynaptic currents. We found that the sums of spine inputs over dendritic trees were counterbalanced by a proportional amount of somatic inhibitory inputs. This online E/I correlation was maintained in dendritic segments that were longer than 50 μm. However, at the single spine level, only 22% of the active spines were activated with inhibitory inputs. This inhibition-coupled activity occurred mainly in the spines with large heads. These results shed light on a microscopic E/I-balancing mechanism that operates at selected synapses and that may increase the accuracy of neural information.

Original languageEnglish
Pages (from-to)1348-1354
Number of pages7
JournalCell Reports
Volume14
Issue number6
DOIs
Publication statusPublished - 2016 Feb 16
Externally publishedYes

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Neurons
Spine
Membranes
Imaging techniques
Inhibitory Postsynaptic Potentials
Pyramidal Cells
Patch-Clamp Techniques
Dendrites
Membrane Potentials
Synapses

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Subcellular Imbalances in Synaptic Activity. / Takahashi, Naoya; Kobayashi, Chiaki; Ishikawa, Tomoe; Ikegaya, Yuji.

In: Cell Reports, Vol. 14, No. 6, 16.02.2016, p. 1348-1354.

Research output: Contribution to journalArticle

Takahashi, N, Kobayashi, C, Ishikawa, T & Ikegaya, Y 2016, 'Subcellular Imbalances in Synaptic Activity', Cell Reports, vol. 14, no. 6, pp. 1348-1354. https://doi.org/10.1016/j.celrep.2016.01.024
Takahashi, Naoya ; Kobayashi, Chiaki ; Ishikawa, Tomoe ; Ikegaya, Yuji. / Subcellular Imbalances in Synaptic Activity. In: Cell Reports. 2016 ; Vol. 14, No. 6. pp. 1348-1354.
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