Subcellular localization of a promoter and an inhibitor of apoptosis (Smac/DIABLO and XIAP) during brain ischemia/reperfusion

Mamoru Shibata, Hidenori Hattori, Takahiro Sasaki, Jun Gotoh, Junichi Hamada, Yasuo Fukuuchi

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

We explored the expression of Smac/DIABLO, a newly identified mitochondrial apoptogenic molecule, and X-linked inhibitor of apoptosis protein (XIAP) in the brain subjected to ischemia/reperfusion. Transient focal ischemia was produced for 1 hour in mice. We observed only a negligible amount of Smac/DIABLO in both mitochondria and cytosol in the normal state. The mitochondrial expression level of Smac/DIABLO increased after 2-11 h reperfusion. There was increased Smac/DIABLO expression in the cytosol after 5 h reperfusion, implying the translocation of Smac/DIABLO into the cytosol. The subcellular localization of XIAP became more extensive within the cells during reperfusion, as compared with the normal state. Our results imply that Smac/DIABLO and XIAP are implicated in the pathophysiological mechanisms of reperfusion injury.

Original languageEnglish
Pages (from-to)1985-1988
Number of pages4
JournalNeuroReport
Volume13
Issue number15
DOIs
Publication statusPublished - 2002 Oct 28

Keywords

  • Caspase-9
  • Cerebral ischemia
  • Cytochrome c
  • Mitochondria
  • Reperfusion
  • Smac/DIABLO
  • XIAP

ASJC Scopus subject areas

  • Neuroscience(all)

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