Subcellular localization of a promoter and an inhibitor of apoptosis (Smac/DIABLO and XIAP) during brain ischemia/reperfusion

Mamoru Shibata; Hidenori Hattori; Takahiro Sasaki; Jun Gotoh; Junichi Hamada; Yasuo Fukuuchi

doi:10.1097/00001756-200210280-00031

Subcellular localization of a promoter and an inhibitor of apoptosis (Smac/DIABLO and XIAP) during brain ischemia/reperfusion

Mamoru Shibata, Hidenori Hattori, Takahiro Sasaki, Jun Gotoh, Junichi Hamada, Yasuo Fukuuchi

Department of Internal Medicine (Neurology)

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

We explored the expression of Smac/DIABLO, a newly identified mitochondrial apoptogenic molecule, and X-linked inhibitor of apoptosis protein (XIAP) in the brain subjected to ischemia/reperfusion. Transient focal ischemia was produced for 1 hour in mice. We observed only a negligible amount of Smac/DIABLO in both mitochondria and cytosol in the normal state. The mitochondrial expression level of Smac/DIABLO increased after 2-11 h reperfusion. There was increased Smac/DIABLO expression in the cytosol after 5 h reperfusion, implying the translocation of Smac/DIABLO into the cytosol. The subcellular localization of XIAP became more extensive within the cells during reperfusion, as compared with the normal state. Our results imply that Smac/DIABLO and XIAP are implicated in the pathophysiological mechanisms of reperfusion injury.

Original language	English
Pages (from-to)	1985-1988
Number of pages	4
Journal	NeuroReport
Volume	13
Issue number	15
DOIs	https://doi.org/10.1097/00001756-200210280-00031
Publication status	Published - 2002 Oct 28

Keywords

Caspase-9
Cerebral ischemia
Cytochrome c
Mitochondria
Reperfusion
Smac/DIABLO
XIAP

ASJC Scopus subject areas

Neuroscience(all)

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10.1097/00001756-200210280-00031

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title = "Subcellular localization of a promoter and an inhibitor of apoptosis (Smac/DIABLO and XIAP) during brain ischemia/reperfusion",

abstract = "We explored the expression of Smac/DIABLO, a newly identified mitochondrial apoptogenic molecule, and X-linked inhibitor of apoptosis protein (XIAP) in the brain subjected to ischemia/reperfusion. Transient focal ischemia was produced for 1 hour in mice. We observed only a negligible amount of Smac/DIABLO in both mitochondria and cytosol in the normal state. The mitochondrial expression level of Smac/DIABLO increased after 2-11 h reperfusion. There was increased Smac/DIABLO expression in the cytosol after 5 h reperfusion, implying the translocation of Smac/DIABLO into the cytosol. The subcellular localization of XIAP became more extensive within the cells during reperfusion, as compared with the normal state. Our results imply that Smac/DIABLO and XIAP are implicated in the pathophysiological mechanisms of reperfusion injury.",

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author = "Mamoru Shibata and Hidenori Hattori and Takahiro Sasaki and Jun Gotoh and Junichi Hamada and Yasuo Fukuuchi",

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T1 - Subcellular localization of a promoter and an inhibitor of apoptosis (Smac/DIABLO and XIAP) during brain ischemia/reperfusion

AU - Shibata, Mamoru

AU - Hattori, Hidenori

AU - Sasaki, Takahiro

AU - Gotoh, Jun

AU - Hamada, Junichi

AU - Fukuuchi, Yasuo

PY - 2002/10/28

Y1 - 2002/10/28

N2 - We explored the expression of Smac/DIABLO, a newly identified mitochondrial apoptogenic molecule, and X-linked inhibitor of apoptosis protein (XIAP) in the brain subjected to ischemia/reperfusion. Transient focal ischemia was produced for 1 hour in mice. We observed only a negligible amount of Smac/DIABLO in both mitochondria and cytosol in the normal state. The mitochondrial expression level of Smac/DIABLO increased after 2-11 h reperfusion. There was increased Smac/DIABLO expression in the cytosol after 5 h reperfusion, implying the translocation of Smac/DIABLO into the cytosol. The subcellular localization of XIAP became more extensive within the cells during reperfusion, as compared with the normal state. Our results imply that Smac/DIABLO and XIAP are implicated in the pathophysiological mechanisms of reperfusion injury.

AB - We explored the expression of Smac/DIABLO, a newly identified mitochondrial apoptogenic molecule, and X-linked inhibitor of apoptosis protein (XIAP) in the brain subjected to ischemia/reperfusion. Transient focal ischemia was produced for 1 hour in mice. We observed only a negligible amount of Smac/DIABLO in both mitochondria and cytosol in the normal state. The mitochondrial expression level of Smac/DIABLO increased after 2-11 h reperfusion. There was increased Smac/DIABLO expression in the cytosol after 5 h reperfusion, implying the translocation of Smac/DIABLO into the cytosol. The subcellular localization of XIAP became more extensive within the cells during reperfusion, as compared with the normal state. Our results imply that Smac/DIABLO and XIAP are implicated in the pathophysiological mechanisms of reperfusion injury.

KW - Caspase-9

KW - Cerebral ischemia

KW - Cytochrome c

KW - Mitochondria

KW - Reperfusion

KW - Smac/DIABLO

KW - XIAP

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U2 - 10.1097/00001756-200210280-00031

DO - 10.1097/00001756-200210280-00031

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AN - SCOPUS:0037191413

SN - 0959-4965

VL - 13

SP - 1985

EP - 1988

JO - NeuroReport

JF - NeuroReport

IS - 15

ER -

Subcellular localization of a promoter and an inhibitor of apoptosis (Smac/DIABLO and XIAP) during brain ischemia/reperfusion

Abstract

Keywords

ASJC Scopus subject areas

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