Subepithelial corneal fibrosis partially due to epithelial-mesenchymal transition of ocular surface epithelium

Motoko Kawashima, Tetsuya Kawakita, Kazunari Higa, Yoshiyuki Satake, Masahiro Omoto, Kazuo Tsubota, Shigeto Shimmura, Jun Shimazaki

Research output: Contribution to journalArticle

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Abstract

Purpose: To determine whether epithelial-mesenchymal transition is involved in the development of corneal subepithelial fibrosis (pannus). Methods: Frozen samples of pannus tissue removed from human corneas with a diagnosis of total limbal stem cell deficiency were characterized by immunostaining for both epithelial and mesenchymal markers. We selected transformation-related protein 63 (p63) and pancytokeratin as epithelial markers and vimentin and α-smooth muscle actin (α-SMA) as mesenchymal markers. Immunostaining for β-catenin and E-cadherin was performed to determine wingless- Int (Wnt)-pathway activation. RT-PCR analysis was also performed on epithelial tissue obtained from pannus samples after dispase digestion. Results: Immunohistochemistry revealed strong nuclear expression of p63 and weak intercellular expression of E-cadherin in epithelial basal cells of pannus tissue. Furthermore, translocation of β-catenin from intercellular junctions to the nucleus and cytoplasm was also observed. Double-positive cells for both p63 and α-SMA were observed in the subepithelial stroma of pannus tissue, which was supported by RT-PCR and cytospin analysis. Conclusions: Epithelial-mesenchymal transition may be partially involved in the development of subepithelial corneal fibrosis due to total limbal stem cell deficiency.

Original languageEnglish
Pages (from-to)2727-2732
Number of pages6
JournalMolecular Vision
Volume16
Publication statusPublished - 2010

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Epithelial-Mesenchymal Transition
Catenins
Fibrosis
Epithelium
Cadherins
Stem Cells
Polymerase Chain Reaction
Wnt Signaling Pathway
Intercellular Junctions
Vimentin
Nuclear Proteins
Cornea
Smooth Muscle
Actins
Digestion
Cytoplasm
Proteins
Epithelial Cells
Immunohistochemistry

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Kawashima, M., Kawakita, T., Higa, K., Satake, Y., Omoto, M., Tsubota, K., ... Shimazaki, J. (2010). Subepithelial corneal fibrosis partially due to epithelial-mesenchymal transition of ocular surface epithelium. Molecular Vision, 16, 2727-2732.

Subepithelial corneal fibrosis partially due to epithelial-mesenchymal transition of ocular surface epithelium. / Kawashima, Motoko; Kawakita, Tetsuya; Higa, Kazunari; Satake, Yoshiyuki; Omoto, Masahiro; Tsubota, Kazuo; Shimmura, Shigeto; Shimazaki, Jun.

In: Molecular Vision, Vol. 16, 2010, p. 2727-2732.

Research output: Contribution to journalArticle

Kawashima, M, Kawakita, T, Higa, K, Satake, Y, Omoto, M, Tsubota, K, Shimmura, S & Shimazaki, J 2010, 'Subepithelial corneal fibrosis partially due to epithelial-mesenchymal transition of ocular surface epithelium', Molecular Vision, vol. 16, pp. 2727-2732.
Kawashima, Motoko ; Kawakita, Tetsuya ; Higa, Kazunari ; Satake, Yoshiyuki ; Omoto, Masahiro ; Tsubota, Kazuo ; Shimmura, Shigeto ; Shimazaki, Jun. / Subepithelial corneal fibrosis partially due to epithelial-mesenchymal transition of ocular surface epithelium. In: Molecular Vision. 2010 ; Vol. 16. pp. 2727-2732.
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AU - Omoto, Masahiro

AU - Tsubota, Kazuo

AU - Shimmura, Shigeto

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