Substrate-induced internalization of the high-affinity choline transporter

Takashi Okuda, Asami Konishi, Hidemi Misawa, Tatsuya Haga

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Cholinergic neurons are endowed with a high-affinity choline uptake system for efficient synthesis of acetylcholine at the presynaptic terminals. The high-affinity choline transporter CHT1 is responsible for choline uptake, the rate-limiting step in acetylcholine synthesis. However, endogenous physiological factors that affect CHT1 expression or function and consequently regulate the acetylcholine synthesis rate are essentially unknown. Here we demonstrate that extracellular substrate decreases the cell-surface expression of CHT1 in rat brain synaptosomes, primary cultures from the basal forebrain, and mammalian cell lines transfected with CHT1. Extracellular choline rapidly decreases cell-surface CHT1 expression by accelerating its internalization, a process that is mediated by a dynamin-dependent endocytosis pathway in HEK293 cells. Specific inhibitor hemicholinium-3 decreases the constitutive internalization rate and thereby increases cell-surface CHT1 expression. We also demonstrate that the constitutive internalization of CHT1 depends on extracellular pH in cultured cells. Our results collectively suggest that the internalization of CHT1 is induced by extracellular substrate, providing a novel feedback mechanism for the regulation of acetylcholine synthesis at the cholinergic presynaptic terminals.

Original languageEnglish
Pages (from-to)14989-14997
Number of pages9
JournalJournal of Neuroscience
Volume31
Issue number42
DOIs
Publication statusPublished - 2011 Oct 19

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Acetylcholine
Choline
Presynaptic Terminals
Hemicholinium 3
Dynamins
Cholinergic Neurons
Synaptosomes
HEK293 Cells
Endocytosis
Cholinergic Agents
Cultured Cells
Cell Line
choline transporter
Brain

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Substrate-induced internalization of the high-affinity choline transporter. / Okuda, Takashi; Konishi, Asami; Misawa, Hidemi; Haga, Tatsuya.

In: Journal of Neuroscience, Vol. 31, No. 42, 19.10.2011, p. 14989-14997.

Research output: Contribution to journalArticle

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