Substrate recognition of renally eliminated angiotensin II receptor blockers by organic anion transporter 4

Saki Noguchi, Moeko Okochi, Hayumi Atsuta, Rika Kimura, Ayaka Fukumoto, Kyoko Takahashi, Tomohiro Nishimura, Masatoshi Tomi

Research output: Contribution to journalArticlepeer-review

Abstract

Organic anion transporter (OAT) 4, which is localized at the apical membrane of human renal proximal tubules, transports olmesartan, an angiotensin II receptor blocker (ARB). Many ARBs, including olmesartan, undergo partial tubular secretion as active forms, and inhibit OAT4-mediated uptake activity. Here, we examined the substrate recognition of various ARBs by OAT4 in order to assess whether OAT4 might be involved in the renal handling of ARBs. Concentration-dependent OAT4-mediated uptake of azilsartan, candesartan, carboxylosartan, losartan, and valsartan was observed with Km values of 6.6, 31, 7.2, 13, and 1.7 μM, respectively, in the absence of extracellular Cl. In the presence of extracellular Cl, OAT4-mediated uptake of dianionic ARBs (azilsartan, candesartan, carboxylosartan, and valsartan) was lower and reached a steady state faster than in the absence of extracellular Cl. Thus, OAT4 is proposed to use extracellular Cl as a counterpart for anion efflux. Our results suggest that OAT4 may play a role in the excretion of azilsartan, candesartan, carboxylosartan, and valsartan, as well as olmesartan. In contrast, OAT4-mediated uptake of losartan, a monoanionic ARB, was little affected by extracellular Cl, suggesting that only OAT4-mediated dianion transport is Cl-sensitive.

Original languageEnglish
Article number100363
JournalDrug Metabolism And Pharmacokinetics
Volume36
DOIs
Publication statusPublished - 2021 Feb

Keywords

  • ARB
  • Angiotensin II receptor Blocker
  • OAT4
  • Organic anion transporter
  • Renal clearance

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science
  • Pharmacology (medical)

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