Substrate specificity of an esterase from the archaeon Sulfolobus tokodaii bearing a GGG(A)X motif

Reina Wada, Masanaru Ozaki, Takashi Kumon, Hiromichi Ohta, Kenji Miyamoto

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A GGG(A)X-type esterase (Est0071) from an archaeon catalyzes asymmetric hydrolysis of prochiral bulky malonic diesters in good enantioselectivity. The selectivity of Est0071 was for the opposite enantiomer to that previously shown for pig liver esterase, and the resulting enantiomeric excess of the products was higher. Est0071 could also catalyze the hydrolysis of various acetates of secondary alcohols, and showed moderate enantioselectivity in these reactions.

Original languageEnglish
Pages (from-to)188-190
Number of pages3
JournalBiocatalysis and Biotransformation
Volume33
Issue number3
DOIs
Publication statusPublished - 2015 May 4

Keywords

  • Esterase
  • GGG(A)X-motif
  • hydrolysis
  • malonic diester
  • Sulfolobus tokodaii

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Catalysis

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