Subventricular zone-derived neuroblasts migrate and differentiate into mature neurons in the post-stroke adult striatum

Toru Yamashita, Mikiko Ninomiya, Pilar Hernández Acosta, Jose Manuel García-Verdugo, Takehiko Sunabori, Masanori Sakaguchi, Kazuhide Adachi, Takuro Kojima, Yuki Hirota, Takeshi Kawase, Nobuo Araki, Koji Abe, Hideyuki Okano, Kazunobu Sawamoto

Research output: Contribution to journalArticle

538 Citations (Scopus)

Abstract

Recent studies have revealed that the adult mammalian brain has the capacity to regenerate some neurons after various insults. However, the precise mechanism of insult-induced neurogenesis has not been demonstrated. In the normal brain, GFAP-expressing cells in the subventricular zone (SVZ) of the lateral ventricles include a neurogenic cell population that gives rise to olfactory bulb neurons only. Herein, we report evidence that, after a stroke, these cells are capable of producing new neurons outside the olfactory bulbs. SVZ GFAP-expressing cells labeled by a cell-type-specific viral infection method were found to generate neuroblasts that migrated toward the injured striatum after middle cerebral artery occlusion. These neuroblasts in the striatum formed elongated chain-like cell aggregates similar to those in the normal SVZ, and these chains were observed to be closely associated with thin astrocytic processes and blood vessels. Finally, long-term tracing of the green fluorescent-labeled cells with a Cre-loxP system revealed that the SVZ-derived neuroblasts differentiated into mature neurons in the striatum, in which they expressed neuronal-specific nuclear protein and formed synapses with neighboring striatal cells. These results highlight the role of the SVZ in neuronal regeneration after a stroke and its potential as an important therapeutic target for various neurological disorders.

Original languageEnglish
Pages (from-to)6627-6636
Number of pages10
JournalJournal of Neuroscience
Volume26
Issue number24
DOIs
Publication statusPublished - 2006

Fingerprint

Lateral Ventricles
Stroke
Neurons
Olfactory Bulb
Corpus Striatum
Middle Cerebral Artery Infarction
Neurogenesis
Brain
Virus Diseases
Nuclear Proteins
Nervous System Diseases
Synapses
Blood Vessels
Regeneration
Population

Keywords

  • Cerebral ischemia
  • Migration
  • Neurogenesis
  • Regeneration
  • Striatum
  • Subventricular zone

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Yamashita, T., Ninomiya, M., Acosta, P. H., García-Verdugo, J. M., Sunabori, T., Sakaguchi, M., ... Sawamoto, K. (2006). Subventricular zone-derived neuroblasts migrate and differentiate into mature neurons in the post-stroke adult striatum. Journal of Neuroscience, 26(24), 6627-6636. https://doi.org/10.1523/JNEUROSCI.0149-06.2006

Subventricular zone-derived neuroblasts migrate and differentiate into mature neurons in the post-stroke adult striatum. / Yamashita, Toru; Ninomiya, Mikiko; Acosta, Pilar Hernández; García-Verdugo, Jose Manuel; Sunabori, Takehiko; Sakaguchi, Masanori; Adachi, Kazuhide; Kojima, Takuro; Hirota, Yuki; Kawase, Takeshi; Araki, Nobuo; Abe, Koji; Okano, Hideyuki; Sawamoto, Kazunobu.

In: Journal of Neuroscience, Vol. 26, No. 24, 2006, p. 6627-6636.

Research output: Contribution to journalArticle

Yamashita, T, Ninomiya, M, Acosta, PH, García-Verdugo, JM, Sunabori, T, Sakaguchi, M, Adachi, K, Kojima, T, Hirota, Y, Kawase, T, Araki, N, Abe, K, Okano, H & Sawamoto, K 2006, 'Subventricular zone-derived neuroblasts migrate and differentiate into mature neurons in the post-stroke adult striatum', Journal of Neuroscience, vol. 26, no. 24, pp. 6627-6636. https://doi.org/10.1523/JNEUROSCI.0149-06.2006
Yamashita, Toru ; Ninomiya, Mikiko ; Acosta, Pilar Hernández ; García-Verdugo, Jose Manuel ; Sunabori, Takehiko ; Sakaguchi, Masanori ; Adachi, Kazuhide ; Kojima, Takuro ; Hirota, Yuki ; Kawase, Takeshi ; Araki, Nobuo ; Abe, Koji ; Okano, Hideyuki ; Sawamoto, Kazunobu. / Subventricular zone-derived neuroblasts migrate and differentiate into mature neurons in the post-stroke adult striatum. In: Journal of Neuroscience. 2006 ; Vol. 26, No. 24. pp. 6627-6636.
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