Successful heterotransplantation of human endometrium in SCID mice

Daisuke Aoki, Yukio Katsuki, Atsushi Shimizu, Chihaya Kakinuma, Shiro Nozawa

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Objective: To establish an experimental system useful for long-term evaluation of human endometrial tissue by its heterotransplantation to SCID mice. Methods: Human endometrium was transplanted subcutaneously into SCID mice, nude mice, or nude mice given anti-asialo GM<inf>1</inf> antibody, a natural killer activity suppressor. The transplant was observed by ultrasonography for up to 10 weeks and then morphologically. To assess the drug susceptibility of the transplant, danazol (10–100 mg/kg/day orally), buserelin (0.01 mg/kg/day subcutaneously), or estradiol (0.5 mg/kg/day intraperitoneally) was administered to the host mice for up to 10 weeks after the transplantation. Multiple-comparison procedures were used for statistical evaluation. Results: The heterotransplant was accepted 100% in SCID mice and natural killer activity-suppressed nude mice, but was significantly inferior (40%) in nude mice at 10 weeks after the transplantation. The transplant size was volumetrically measurable noninvasively by ultrasonography. Using this SCID mouse model, we could evaluate drug effects on the transplanted endometrium. Conclusion: This system in SCID mice may be useful for evaluating drug actions on human endometrium and endometriotic tissues.

Original languageEnglish
Pages (from-to)220-228
Number of pages9
JournalObstetrics and Gynecology
Volume83
Issue number2
Publication statusPublished - 1994

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SCID Mice
Endometrium
Nude Mice
Transplants
Ultrasonography
Transplantation
Pharmaceutical Preparations
Buserelin
Danazol
Heterografts
Estradiol
Antibodies

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Aoki, D., Katsuki, Y., Shimizu, A., Kakinuma, C., & Nozawa, S. (1994). Successful heterotransplantation of human endometrium in SCID mice. Obstetrics and Gynecology, 83(2), 220-228.

Successful heterotransplantation of human endometrium in SCID mice. / Aoki, Daisuke; Katsuki, Yukio; Shimizu, Atsushi; Kakinuma, Chihaya; Nozawa, Shiro.

In: Obstetrics and Gynecology, Vol. 83, No. 2, 1994, p. 220-228.

Research output: Contribution to journalArticle

Aoki, D, Katsuki, Y, Shimizu, A, Kakinuma, C & Nozawa, S 1994, 'Successful heterotransplantation of human endometrium in SCID mice', Obstetrics and Gynecology, vol. 83, no. 2, pp. 220-228.
Aoki D, Katsuki Y, Shimizu A, Kakinuma C, Nozawa S. Successful heterotransplantation of human endometrium in SCID mice. Obstetrics and Gynecology. 1994;83(2):220-228.
Aoki, Daisuke ; Katsuki, Yukio ; Shimizu, Atsushi ; Kakinuma, Chihaya ; Nozawa, Shiro. / Successful heterotransplantation of human endometrium in SCID mice. In: Obstetrics and Gynecology. 1994 ; Vol. 83, No. 2. pp. 220-228.
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