Suction effusion fluid from skin and constituent analysis: New candidate for interstitial fluid

S. Kayashima, Tsunenori Arai, M. Kikuchi, N. Nagata, N. Ito, T. Kuriyama, J. Kimura

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The authors analyzed the constituents of effuse transcutaneous fluid, labeled suction effusion fluid (SEF), and monitored its glucose concentration during glucose loading in rabbits (Japan White, female) under pentobarbital anesthesia. The SEF was sampled by suctioning corneal layer-stripped skin at 400 mmHg absolute pressure. The SEF proved to have nearly the same concentrations as serum for lower-molecular-weight substances such as glucose, creatinine, and urea nitrogens, but not for higher-molecular-weight substances such as serum proteins. The SEF protein concentration was one- fourth that of serum protein. Proteins >100 kDa molecular mass were barely detectable in the SEF. Monitoring of SEF glucose change every 10 min during intravenous glucose loading was successfully accomplished, and SEF glucose concentration followed blood glucose concentration with a 10-min delay. The SEF was thought to consist of interstitial fluid and/or effuse fluid from small vessels in subcutaneous tissue.

Original languageEnglish
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume263
Issue number5 32-5
Publication statusPublished - 1992
Externally publishedYes

Fingerprint

Extracellular Fluid
Suction
Skin
Glucose
Blood Proteins
Molecular Weight
Subcutaneous Tissue
Pentobarbital
Blood Glucose
Urea
Creatinine
Japan
Proteins
Nitrogen
Anesthesia
Rabbits
Pressure
Serum

Keywords

  • glucose measurement
  • interstitium
  • rabbit
  • transcutaneous method

ASJC Scopus subject areas

  • Physiology

Cite this

Kayashima, S., Arai, T., Kikuchi, M., Nagata, N., Ito, N., Kuriyama, T., & Kimura, J. (1992). Suction effusion fluid from skin and constituent analysis: New candidate for interstitial fluid. American Journal of Physiology - Heart and Circulatory Physiology, 263(5 32-5).

Suction effusion fluid from skin and constituent analysis : New candidate for interstitial fluid. / Kayashima, S.; Arai, Tsunenori; Kikuchi, M.; Nagata, N.; Ito, N.; Kuriyama, T.; Kimura, J.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 263, No. 5 32-5, 1992.

Research output: Contribution to journalArticle

Kayashima, S, Arai, T, Kikuchi, M, Nagata, N, Ito, N, Kuriyama, T & Kimura, J 1992, 'Suction effusion fluid from skin and constituent analysis: New candidate for interstitial fluid', American Journal of Physiology - Heart and Circulatory Physiology, vol. 263, no. 5 32-5.
Kayashima, S. ; Arai, Tsunenori ; Kikuchi, M. ; Nagata, N. ; Ito, N. ; Kuriyama, T. ; Kimura, J. / Suction effusion fluid from skin and constituent analysis : New candidate for interstitial fluid. In: American Journal of Physiology - Heart and Circulatory Physiology. 1992 ; Vol. 263, No. 5 32-5.
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