Sulfatide inhibits α-galactosylceramide presentation by dendritic cells

Mitsuhiro Kanamori, Yusuke Tasumi, Tomonori Iyoda, Maki Ushida, Kayo Inaba

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Sulfatide-reactive type II NKT cells, the so-called non-invariant NKT (non-iNKT) cells, have been shown to counteract invariant NKT (iNKT) cell activity. However, the effects of sulfatide on activation of iNKT cells by α-galactocylceramide (αGC) in the context of CD1d have not been studied in detail. Therefore, we studied the blocking effect of sulfatide on αGC-induced iNKT cell activation by dendritic cells (DCs). Even in the absence of non-iNKT cells, sulfatide inhibited αGC-mediated iNKT cell activation by reducing αGC/CD1d complex formations in a dose-dependent manner. This was also confirmed in a cell-free setting using immobilized CD1d-Ig. Moreover, simultaneous injection of αGC with sulfatide decreased αGC/CD1d complex formations on DCs, accompanied by the reduced CD40L-up-regulation and IFN-γ production by iNKT cells and IL-12p70 production by DCs. However, sulfatide by itself did not interfere with the presentation of MHC class II-mediated antigen presentation to specific T cells. These results demonstrate that sulfatide competes with αGC to be loaded onto CD1d along the endocytic pathway in DCs, thereby inhibiting the iNKT cell response.

Original languageEnglish
Pages (from-to)129-136
Number of pages8
JournalInternational Immunology
Volume24
Issue number2
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Galactosylceramides
Sulfoglycosphingolipids
Natural Killer T-Cells
Dendritic Cells
CD40 Ligand
Histocompatibility Antigens Class II
Antigen Presentation
Up-Regulation
T-Lymphocytes

Keywords

  • α-GalCer
  • CD1d
  • Dendritic cell
  • NKT cell

ASJC Scopus subject areas

  • Immunology

Cite this

Kanamori, M., Tasumi, Y., Iyoda, T., Ushida, M., & Inaba, K. (2012). Sulfatide inhibits α-galactosylceramide presentation by dendritic cells. International Immunology, 24(2), 129-136. https://doi.org/10.1093/intimm/dxr108

Sulfatide inhibits α-galactosylceramide presentation by dendritic cells. / Kanamori, Mitsuhiro; Tasumi, Yusuke; Iyoda, Tomonori; Ushida, Maki; Inaba, Kayo.

In: International Immunology, Vol. 24, No. 2, 2012, p. 129-136.

Research output: Contribution to journalArticle

Kanamori, M, Tasumi, Y, Iyoda, T, Ushida, M & Inaba, K 2012, 'Sulfatide inhibits α-galactosylceramide presentation by dendritic cells', International Immunology, vol. 24, no. 2, pp. 129-136. https://doi.org/10.1093/intimm/dxr108
Kanamori, Mitsuhiro ; Tasumi, Yusuke ; Iyoda, Tomonori ; Ushida, Maki ; Inaba, Kayo. / Sulfatide inhibits α-galactosylceramide presentation by dendritic cells. In: International Immunology. 2012 ; Vol. 24, No. 2. pp. 129-136.
@article{5c5be6db30cc442ea1e9764910f12d52,
title = "Sulfatide inhibits α-galactosylceramide presentation by dendritic cells",
abstract = "Sulfatide-reactive type II NKT cells, the so-called non-invariant NKT (non-iNKT) cells, have been shown to counteract invariant NKT (iNKT) cell activity. However, the effects of sulfatide on activation of iNKT cells by α-galactocylceramide (αGC) in the context of CD1d have not been studied in detail. Therefore, we studied the blocking effect of sulfatide on αGC-induced iNKT cell activation by dendritic cells (DCs). Even in the absence of non-iNKT cells, sulfatide inhibited αGC-mediated iNKT cell activation by reducing αGC/CD1d complex formations in a dose-dependent manner. This was also confirmed in a cell-free setting using immobilized CD1d-Ig. Moreover, simultaneous injection of αGC with sulfatide decreased αGC/CD1d complex formations on DCs, accompanied by the reduced CD40L-up-regulation and IFN-γ production by iNKT cells and IL-12p70 production by DCs. However, sulfatide by itself did not interfere with the presentation of MHC class II-mediated antigen presentation to specific T cells. These results demonstrate that sulfatide competes with αGC to be loaded onto CD1d along the endocytic pathway in DCs, thereby inhibiting the iNKT cell response.",
keywords = "α-GalCer, CD1d, Dendritic cell, NKT cell",
author = "Mitsuhiro Kanamori and Yusuke Tasumi and Tomonori Iyoda and Maki Ushida and Kayo Inaba",
year = "2012",
doi = "10.1093/intimm/dxr108",
language = "English",
volume = "24",
pages = "129--136",
journal = "International Immunology",
issn = "0953-8178",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - Sulfatide inhibits α-galactosylceramide presentation by dendritic cells

AU - Kanamori, Mitsuhiro

AU - Tasumi, Yusuke

AU - Iyoda, Tomonori

AU - Ushida, Maki

AU - Inaba, Kayo

PY - 2012

Y1 - 2012

N2 - Sulfatide-reactive type II NKT cells, the so-called non-invariant NKT (non-iNKT) cells, have been shown to counteract invariant NKT (iNKT) cell activity. However, the effects of sulfatide on activation of iNKT cells by α-galactocylceramide (αGC) in the context of CD1d have not been studied in detail. Therefore, we studied the blocking effect of sulfatide on αGC-induced iNKT cell activation by dendritic cells (DCs). Even in the absence of non-iNKT cells, sulfatide inhibited αGC-mediated iNKT cell activation by reducing αGC/CD1d complex formations in a dose-dependent manner. This was also confirmed in a cell-free setting using immobilized CD1d-Ig. Moreover, simultaneous injection of αGC with sulfatide decreased αGC/CD1d complex formations on DCs, accompanied by the reduced CD40L-up-regulation and IFN-γ production by iNKT cells and IL-12p70 production by DCs. However, sulfatide by itself did not interfere with the presentation of MHC class II-mediated antigen presentation to specific T cells. These results demonstrate that sulfatide competes with αGC to be loaded onto CD1d along the endocytic pathway in DCs, thereby inhibiting the iNKT cell response.

AB - Sulfatide-reactive type II NKT cells, the so-called non-invariant NKT (non-iNKT) cells, have been shown to counteract invariant NKT (iNKT) cell activity. However, the effects of sulfatide on activation of iNKT cells by α-galactocylceramide (αGC) in the context of CD1d have not been studied in detail. Therefore, we studied the blocking effect of sulfatide on αGC-induced iNKT cell activation by dendritic cells (DCs). Even in the absence of non-iNKT cells, sulfatide inhibited αGC-mediated iNKT cell activation by reducing αGC/CD1d complex formations in a dose-dependent manner. This was also confirmed in a cell-free setting using immobilized CD1d-Ig. Moreover, simultaneous injection of αGC with sulfatide decreased αGC/CD1d complex formations on DCs, accompanied by the reduced CD40L-up-regulation and IFN-γ production by iNKT cells and IL-12p70 production by DCs. However, sulfatide by itself did not interfere with the presentation of MHC class II-mediated antigen presentation to specific T cells. These results demonstrate that sulfatide competes with αGC to be loaded onto CD1d along the endocytic pathway in DCs, thereby inhibiting the iNKT cell response.

KW - α-GalCer

KW - CD1d

KW - Dendritic cell

KW - NKT cell

UR - http://www.scopus.com/inward/record.url?scp=84856588122&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84856588122&partnerID=8YFLogxK

U2 - 10.1093/intimm/dxr108

DO - 10.1093/intimm/dxr108

M3 - Article

C2 - 22247226

AN - SCOPUS:84856588122

VL - 24

SP - 129

EP - 136

JO - International Immunology

JF - International Immunology

SN - 0953-8178

IS - 2

ER -