31P-NMR study of the physiological conditions in intact root cells of mung bean seedlings under low-temperature stress

Yuichi Takeda, Tomoko Ogawa, Yoshiyuki Nakamura, Kunihiro Kasamo, Makoto Sakata, Eiji Ohta

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Intracellular pH and levels of ATP in intact root-tip cells of mung bean (Vigna mungo [L.] Hepper) under low-temperature stress were investigated in vivo by 31P nuclear magnetic resonance (31P-NMR) spectroscopy. Root-tips of 3 mm in length were excised from seedlings of mung bean that had been chilled at 0°C after grown at 30°C. Chilling for longer than 12 h caused changes in the intracellular pH and decreased levels of ATP in the seedlings. The level of ATP recovered within 30 min but little change in pH was observed when samples were rewarmed to 20° C after chilling at 5°C. However, after chilling for longer than 48 h, neither the intracellular pH nor the level of ATP was restored.These results suggest that a decline in the activity of tonoplast H+-ATPase, induced by chillings, might be a significant early event in cold-induced injury that leads to cell damage.

Original languageEnglish
Pages (from-to)865-871
Number of pages7
JournalPlant and Cell Physiology
Volume36
Issue number5
Publication statusPublished - 1995 Jul

Fingerprint

Mung Bean
Chilling
Nuclear Magnetic Resonance
Adenosinetriphosphate
mung beans
Seedlings
nuclear magnetic resonance
nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy
Adenosine Triphosphate
Nuclear magnetic resonance
seedling
Roots
Temperature
Meristem
seedlings
Cell
root tips
temperature
spectroscopy

Keywords

  • 31P nuclear magnetic resonance
  • Cold-induced injury
  • Intracellular pH
  • Level of ATP
  • Low-temperature stress
  • Mung bean {Vigna mungo)

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

Takeda, Y., Ogawa, T., Nakamura, Y., Kasamo, K., Sakata, M., & Ohta, E. (1995). 31P-NMR study of the physiological conditions in intact root cells of mung bean seedlings under low-temperature stress. Plant and Cell Physiology, 36(5), 865-871.

31P-NMR study of the physiological conditions in intact root cells of mung bean seedlings under low-temperature stress. / Takeda, Yuichi; Ogawa, Tomoko; Nakamura, Yoshiyuki; Kasamo, Kunihiro; Sakata, Makoto; Ohta, Eiji.

In: Plant and Cell Physiology, Vol. 36, No. 5, 07.1995, p. 865-871.

Research output: Contribution to journalArticle

Takeda, Y, Ogawa, T, Nakamura, Y, Kasamo, K, Sakata, M & Ohta, E 1995, '31P-NMR study of the physiological conditions in intact root cells of mung bean seedlings under low-temperature stress', Plant and Cell Physiology, vol. 36, no. 5, pp. 865-871.
Takeda, Yuichi ; Ogawa, Tomoko ; Nakamura, Yoshiyuki ; Kasamo, Kunihiro ; Sakata, Makoto ; Ohta, Eiji. / 31P-NMR study of the physiological conditions in intact root cells of mung bean seedlings under low-temperature stress. In: Plant and Cell Physiology. 1995 ; Vol. 36, No. 5. pp. 865-871.
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