Superoxide inhibits neuronal nitric oxide synthase influences on afferent arterioles in spontaneously hypertensive rats

Atsuhiro Ichihara, Matsuhiko Hayashi, Nobuhisa Hirota, Takao Saruta

Research output: Contribution to journalArticle

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Abstract

This study was designed to determine the influence of increased superoxide anion in neuronal nitric oxide synthase (nNOS)-dependent regulation of afferent arterioles in spontaneously hypertensive rats (SHR). Afferent arteriolar diameters of male Wistar-Kyoto rats (WKY) and SHR were assessed in vitro with the blood-perfused juxtamedullary nephron technique and averaged 21.6±1.6 (n=6) and 18.8±1.2 (n=7) μm, respectively. The superoxide dismutase mimetic Tempol (1, 10, and 100 μmol/L) did not influence afferent arterioles of WKY but significantly increased afferent arteriolar diameters of SHR by 20.6±5.5%, 25.2±5.4%, and 23.3±4.9%, respectively. In WKY (n=6), the nNOS inhibitor S-methyl-L-thiocitrulline (L-SMTC: 10 μmol/L) and the NOS inhibitor Nω-nitro-L-arginine (L-NNA; 100 μmol/L) significantly decreased afferent arteriolar diameters (19.6±1.6 μm) by 11.9±3.1% and 21.0±3.9%, respectively. In SHR (n=7), L-SMTC did not influence afferent arteriolar diameters (21.0±1.5 μm), but L-NNA exerted an afferent arteriolar constriction (14.8±3.2%) that was similar to the response observed in WKY. Experiments were also performed in the presence of 100μmol/L Tempol. In afferent arterioles of WKY (n=6). Tempol treatment did not modulate the basal diameters (21.5±1.2 μm) or the constrictor response to L-SMTC (10.6±2.1%) or L-NNA (19.3±3.3%). In SHR (n=8), Tempol significantly increased afferent arteriolar diameters by 22.5±4.3% and enhanced afferent arteriolar constrictor responses to L-SMTC (18.4±2.7%) and L-NNA (31.9±2.6%). However, the nitric oxide donor S-nitroso-N-acetylpenicillamine (10μmol/L), which similarly increased afferent arteriolar diameters (17.2±2.3%, n=6), did not affect afferent arteriolar responses to L-SMTC (1.5±2.7%) or L-NNA (18.6±2.3%). These suggest that superoxide anion inhibits the control of afferent arteriolar diameters by nNOS in SHR.

Original languageEnglish
Pages (from-to)630-634
Number of pages5
JournalHypertension
Volume37
Issue number2 II
Publication statusPublished - 2001

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Nitric Oxide Synthase Type I
Arterioles
Inbred SHR Rats
Inbred WKY Rats
Superoxides
S-Nitroso-N-Acetylpenicillamine
Nitric Oxide Donors
Nephrons
Constriction
Superoxide Dismutase
Arginine
tempol

Keywords

  • Arterioles
  • Kidney
  • Nitric oxide synthase
  • Rats
  • Spontaneously hypertensive
  • Tempol

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Superoxide inhibits neuronal nitric oxide synthase influences on afferent arterioles in spontaneously hypertensive rats. / Ichihara, Atsuhiro; Hayashi, Matsuhiko; Hirota, Nobuhisa; Saruta, Takao.

In: Hypertension, Vol. 37, No. 2 II, 2001, p. 630-634.

Research output: Contribution to journalArticle

Ichihara, A, Hayashi, M, Hirota, N & Saruta, T 2001, 'Superoxide inhibits neuronal nitric oxide synthase influences on afferent arterioles in spontaneously hypertensive rats', Hypertension, vol. 37, no. 2 II, pp. 630-634.
Ichihara, Atsuhiro ; Hayashi, Matsuhiko ; Hirota, Nobuhisa ; Saruta, Takao. / Superoxide inhibits neuronal nitric oxide synthase influences on afferent arterioles in spontaneously hypertensive rats. In: Hypertension. 2001 ; Vol. 37, No. 2 II. pp. 630-634.
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N2 - This study was designed to determine the influence of increased superoxide anion in neuronal nitric oxide synthase (nNOS)-dependent regulation of afferent arterioles in spontaneously hypertensive rats (SHR). Afferent arteriolar diameters of male Wistar-Kyoto rats (WKY) and SHR were assessed in vitro with the blood-perfused juxtamedullary nephron technique and averaged 21.6±1.6 (n=6) and 18.8±1.2 (n=7) μm, respectively. The superoxide dismutase mimetic Tempol (1, 10, and 100 μmol/L) did not influence afferent arterioles of WKY but significantly increased afferent arteriolar diameters of SHR by 20.6±5.5%, 25.2±5.4%, and 23.3±4.9%, respectively. In WKY (n=6), the nNOS inhibitor S-methyl-L-thiocitrulline (L-SMTC: 10 μmol/L) and the NOS inhibitor Nω-nitro-L-arginine (L-NNA; 100 μmol/L) significantly decreased afferent arteriolar diameters (19.6±1.6 μm) by 11.9±3.1% and 21.0±3.9%, respectively. In SHR (n=7), L-SMTC did not influence afferent arteriolar diameters (21.0±1.5 μm), but L-NNA exerted an afferent arteriolar constriction (14.8±3.2%) that was similar to the response observed in WKY. Experiments were also performed in the presence of 100μmol/L Tempol. In afferent arterioles of WKY (n=6). Tempol treatment did not modulate the basal diameters (21.5±1.2 μm) or the constrictor response to L-SMTC (10.6±2.1%) or L-NNA (19.3±3.3%). In SHR (n=8), Tempol significantly increased afferent arteriolar diameters by 22.5±4.3% and enhanced afferent arteriolar constrictor responses to L-SMTC (18.4±2.7%) and L-NNA (31.9±2.6%). However, the nitric oxide donor S-nitroso-N-acetylpenicillamine (10μmol/L), which similarly increased afferent arteriolar diameters (17.2±2.3%, n=6), did not affect afferent arteriolar responses to L-SMTC (1.5±2.7%) or L-NNA (18.6±2.3%). These suggest that superoxide anion inhibits the control of afferent arteriolar diameters by nNOS in SHR.

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KW - Spontaneously hypertensive

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