Support of early B-cell differentiation in mouse fetal liver by stromal cells and interleukin-7

Yuji Gunji, Tetsuo Sudo, Junko Suda, Yuji Yamaguchi, Hiromitsu Nakauchi, Shin Ichi Nishikawa, Nobuyuki Yanai, Masuo Obinata, Masayoshi Yanagisawa, Yasusada Miura, Toshio Suda

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28 Citations (Scopus)

Abstract

We compared the development of B-cell progenitors with that of myeloid progenitors in fetal liver cells at various gestational ages. Day 12 to 14 fetal liver cells did not form pre-B-cell colonies. Pre-B-cell colonies were developed from day 15 fetal liver cells. The incidence of colonies increased with increases in gestational age and reached a maximum on days 18 to 19. In contrast, the incidence of myeloid colonies formed in the presence of interleukin-3 (IL-3) and erythropoietin did not change significantly during days 13 to 21 of gestation. After coculturing day 13 fetal liver cells with IL-7-producing stromal cell line ST-2, they could respond to IL-7 and proliferate. Analysis of the phenotypes showed that day 13 fetal liver cells were B220-, IgM-, while culturing day 13 fetal liver cells with ST-2 and untreated day 18 fetal liver cells contained the population of B220 cells. Even in the presence of IL-7-defective stromal cell line FLS-3, IL-7-responsive cells could be induced from day 13 fetal liver cells. IL-7 acted on B220+ cells and induced pre-B-cell colonies that contained IgM+ cells in the methylcellulose culture. IL-7 mRNA was expressed in days 13 and 18 fetal liver cells but not in pre-B cells or adult liver cells. From these findings, it is suggested that stromal cells or stromal-derived factors but not IL-7 were required for the differentiation from B220- cells to B220+ cells. In the second stage, B220+, IgM- cells proliferated and some of them differentiated to IgM+ cells in the presence of IL-7 alone. The two-step model can apply to in vivo early B lymphopoiesis.

Original languageEnglish
Pages (from-to)2612-2617
Number of pages6
JournalBlood
Volume77
Issue number12
Publication statusPublished - 1991 Jun 15

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Interleukin-7
Stromal Cells
Liver
Cell Differentiation
B-Lymphocytes
Cells
Immunoglobulin M
B-Lymphoid Precursor Cells
Methylcellulose
Interleukin-3
Gestational Age
Erythropoietin
Lymphopoiesis
Cell Line
Incidence

ASJC Scopus subject areas

  • Hematology

Cite this

Gunji, Y., Sudo, T., Suda, J., Yamaguchi, Y., Nakauchi, H., Nishikawa, S. I., ... Suda, T. (1991). Support of early B-cell differentiation in mouse fetal liver by stromal cells and interleukin-7. Blood, 77(12), 2612-2617.

Support of early B-cell differentiation in mouse fetal liver by stromal cells and interleukin-7. / Gunji, Yuji; Sudo, Tetsuo; Suda, Junko; Yamaguchi, Yuji; Nakauchi, Hiromitsu; Nishikawa, Shin Ichi; Yanai, Nobuyuki; Obinata, Masuo; Yanagisawa, Masayoshi; Miura, Yasusada; Suda, Toshio.

In: Blood, Vol. 77, No. 12, 15.06.1991, p. 2612-2617.

Research output: Contribution to journalArticle

Gunji, Y, Sudo, T, Suda, J, Yamaguchi, Y, Nakauchi, H, Nishikawa, SI, Yanai, N, Obinata, M, Yanagisawa, M, Miura, Y & Suda, T 1991, 'Support of early B-cell differentiation in mouse fetal liver by stromal cells and interleukin-7', Blood, vol. 77, no. 12, pp. 2612-2617.
Gunji Y, Sudo T, Suda J, Yamaguchi Y, Nakauchi H, Nishikawa SI et al. Support of early B-cell differentiation in mouse fetal liver by stromal cells and interleukin-7. Blood. 1991 Jun 15;77(12):2612-2617.
Gunji, Yuji ; Sudo, Tetsuo ; Suda, Junko ; Yamaguchi, Yuji ; Nakauchi, Hiromitsu ; Nishikawa, Shin Ichi ; Yanai, Nobuyuki ; Obinata, Masuo ; Yanagisawa, Masayoshi ; Miura, Yasusada ; Suda, Toshio. / Support of early B-cell differentiation in mouse fetal liver by stromal cells and interleukin-7. In: Blood. 1991 ; Vol. 77, No. 12. pp. 2612-2617.
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AU - Nishikawa, Shin Ichi

AU - Yanai, Nobuyuki

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