TY - JOUR
T1 - Suppression by Cannabinoids of a Cloned Cell Line with Natural Killer Cell Activity
AU - Kawakami, Yutaka
AU - Klein, Thomas W.
AU - Newton, Catherine
AU - Djeu, Julie Y.
AU - Specter, Steven
AU - Friedman, Herman
AU - Dennert, Gunther
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1988/3
Y1 - 1988/3
N2 - Preincubation of a cloned cell line with natural killer (NK) cell activity, as well as splenic mononuclear cells with either Δ9-tetrahydrocannabinol (THC) or 11-hydroxy-Δ9-tetrahydrocannabinol (11-OH-THC) suppressed NK cytolytic activity against YAC-1 target cells in a dose-dependent manner. THC was more inhibitory for cloned cells than 11-OH-THC and suppressed the lytic activity of these cells without reducing cell viability in the concentration range of 5 μg/ml (16 μM) to 10 μg/ml (32 μM). THC also inhibited proliferation of cloned NK cells, but this inhibitory effect was reversible in that extensive washing of cells following cannabinoid pretreatment eliminated the suppressive effect. Single-cell analysis revealed that THC did not inhibit the binding of cloned NK cells to target cells and further showed that NK cells freshly isolated from mouse spleen were restricted in killing capacity following binding to target cells. Therefore, THC and 11-OH-THC appear to directly inhibit NK cell cytolytic activity in a postbinding stage.
AB - Preincubation of a cloned cell line with natural killer (NK) cell activity, as well as splenic mononuclear cells with either Δ9-tetrahydrocannabinol (THC) or 11-hydroxy-Δ9-tetrahydrocannabinol (11-OH-THC) suppressed NK cytolytic activity against YAC-1 target cells in a dose-dependent manner. THC was more inhibitory for cloned cells than 11-OH-THC and suppressed the lytic activity of these cells without reducing cell viability in the concentration range of 5 μg/ml (16 μM) to 10 μg/ml (32 μM). THC also inhibited proliferation of cloned NK cells, but this inhibitory effect was reversible in that extensive washing of cells following cannabinoid pretreatment eliminated the suppressive effect. Single-cell analysis revealed that THC did not inhibit the binding of cloned NK cells to target cells and further showed that NK cells freshly isolated from mouse spleen were restricted in killing capacity following binding to target cells. Therefore, THC and 11-OH-THC appear to directly inhibit NK cell cytolytic activity in a postbinding stage.
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U2 - 10.3181/00379727-187-42676
DO - 10.3181/00379727-187-42676
M3 - Article
C2 - 2831542
AN - SCOPUS:0023838835
SN - 0037-9727
VL - 187
SP - 355
EP - 359
JO - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
JF - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
IS - 3
ER -