Suppression by Cannabinoids of a Cloned Cell Line with Natural Killer Cell Activity

Yutaka Kawakami, Thomas W. Klein, Catherine Newton, Julie Y. Djeu, Steven Specter, Herman Friedman, Gunther Dennert

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Preincubation of a cloned cell line with natural killer (NK) cell activity, as well as splenic mononuclear cells with either Δ9-tetrahydrocannabinol (THC) or 11-hydroxy-Δ9-tetrahydrocannabinol (11-OH-THC) suppressed NK cytolytic activity against YAC-1 target cells in a dose-dependent manner. THC was more inhibitory for cloned cells than 11-OH-THC and suppressed the lytic activity of these cells without reducing cell viability in the concentration range of 5 μg/ml (16 μM) to 10 μg/ml (32 μM). THC also inhibited proliferation of cloned NK cells, but this inhibitory effect was reversible in that extensive washing of cells following cannabinoid pretreatment eliminated the suppressive effect. Single-cell analysis revealed that THC did not inhibit the binding of cloned NK cells to target cells and further showed that NK cells freshly isolated from mouse spleen were restricted in killing capacity following binding to target cells. Therefore, THC and 11-OH-THC appear to directly inhibit NK cell cytolytic activity in a postbinding stage.

Original languageEnglish
Pages (from-to)355-359
Number of pages5
JournalProceedings of the Society for Experimental Biology and Medicine
Volume187
Issue number3
DOIs
Publication statusPublished - 1988 Mar

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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