Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2

Hiroshi Mochimaru, Tomohiko Usui, Tomonori Yaguchi, Yasuharu Nagahama, Go Hasegawa, Yoshihiko Usui, Shigeto Shimmura, Kazuo Tsubota, Shiro Amano, Yutaka Kawakami, Susumu Ishida

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Abstract

Purpose. To investigate whether the induction of cytotoxic T lymphocytes (CTLs) targeting VEGF receptor 2 inhibits corneal neovascularization caused by alkali injury. Methods. H-2Db-restricted peptide corresponding to amino acids 400 to 408 of VEGF receptor 2 (VEGFR2400-408) was used as an epitope peptide. Dendritic cells (DCs) were harvested from bone marrow progenitors of C57BL/6 mice. Six- week-old C57BL/6 mice received subcutaneous injections of VEGFR2400-408- or gp70-pulsed mature DCs three times at 6-day intervals. After the third immunization, corneal neovas- cularization was induced by alkali injury. Two weeks after the injury, the corneal vascularized area was evaluated by lectin angiography. To confirm the peptide-specific CTL activities in C57BL/6 mice, CD8+ T cells from immunized mice were subjected to ELISA for interferon (IFN)-γ and tumor necrosis factor (TNF)-α production and 51Cr-release cytotoxicity assay. To determine the in vivo effector T cells, the immunized mice were intraperitoneally injected with an anti-CD4 or -CD8 depletion antibody. Results. Corneal neovascularization was significantly attenuated in mice immunized with VEGFR2400-408 compared with those not immunized or immunized with gp70. VEGFR2400-408 or gp70, but not β-gal96-103 application led to dose-dependent induction of IFN-γ and TNF-α in the CD8+ T cells cocultured with stimulator cells. Cytotoxicity assays showed the specific lysis of major histocompatibility complex-matched cells expressing VEGFR2, but not β-gal96-103. In vivo depletion of CD8+, but not CD4+, T cells significantly reversed the suppressive effect of VEGFR2400-408 immunization on corneal neovascularization to the level observed in nonimmunized or gp70-immunized animals. Conclusions. These results indicate the possibility of DC vaccination targeting VEGFR2 as a novel therapeutic strategy for corneal chemical injury.

Original languageEnglish
Pages (from-to)2172-2177
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume49
Issue number5
DOIs
Publication statusPublished - 2008 Apr

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Corneal Neovascularization
Vascular Endothelial Growth Factor Receptor
Alkalies
Burns
Dendritic Cells
Vaccination
Inbred C57BL Mouse
T-Lymphocytes
Cytotoxic T-Lymphocytes
Interferons
Peptides
Immunization
Tumor Necrosis Factor-alpha
Wounds and Injuries
Subcutaneous Injections
Major Histocompatibility Complex
Lectins
Epitopes
Angiography
Bone Marrow

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2. / Mochimaru, Hiroshi; Usui, Tomohiko; Yaguchi, Tomonori; Nagahama, Yasuharu; Hasegawa, Go; Usui, Yoshihiko; Shimmura, Shigeto; Tsubota, Kazuo; Amano, Shiro; Kawakami, Yutaka; Ishida, Susumu.

In: Investigative Ophthalmology and Visual Science, Vol. 49, No. 5, 04.2008, p. 2172-2177.

Research output: Contribution to journalArticle

Mochimaru, Hiroshi ; Usui, Tomohiko ; Yaguchi, Tomonori ; Nagahama, Yasuharu ; Hasegawa, Go ; Usui, Yoshihiko ; Shimmura, Shigeto ; Tsubota, Kazuo ; Amano, Shiro ; Kawakami, Yutaka ; Ishida, Susumu. / Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2. In: Investigative Ophthalmology and Visual Science. 2008 ; Vol. 49, No. 5. pp. 2172-2177.
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abstract = "Purpose. To investigate whether the induction of cytotoxic T lymphocytes (CTLs) targeting VEGF receptor 2 inhibits corneal neovascularization caused by alkali injury. Methods. H-2Db-restricted peptide corresponding to amino acids 400 to 408 of VEGF receptor 2 (VEGFR2400-408) was used as an epitope peptide. Dendritic cells (DCs) were harvested from bone marrow progenitors of C57BL/6 mice. Six- week-old C57BL/6 mice received subcutaneous injections of VEGFR2400-408- or gp70-pulsed mature DCs three times at 6-day intervals. After the third immunization, corneal neovas- cularization was induced by alkali injury. Two weeks after the injury, the corneal vascularized area was evaluated by lectin angiography. To confirm the peptide-specific CTL activities in C57BL/6 mice, CD8+ T cells from immunized mice were subjected to ELISA for interferon (IFN)-γ and tumor necrosis factor (TNF)-α production and 51Cr-release cytotoxicity assay. To determine the in vivo effector T cells, the immunized mice were intraperitoneally injected with an anti-CD4 or -CD8 depletion antibody. Results. Corneal neovascularization was significantly attenuated in mice immunized with VEGFR2400-408 compared with those not immunized or immunized with gp70. VEGFR2400-408 or gp70, but not β-gal96-103 application led to dose-dependent induction of IFN-γ and TNF-α in the CD8+ T cells cocultured with stimulator cells. Cytotoxicity assays showed the specific lysis of major histocompatibility complex-matched cells expressing VEGFR2, but not β-gal96-103. In vivo depletion of CD8+, but not CD4+, T cells significantly reversed the suppressive effect of VEGFR2400-408 immunization on corneal neovascularization to the level observed in nonimmunized or gp70-immunized animals. Conclusions. These results indicate the possibility of DC vaccination targeting VEGFR2 as a novel therapeutic strategy for corneal chemical injury.",
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AU - Nagahama, Yasuharu

AU - Hasegawa, Go

AU - Usui, Yoshihiko

AU - Shimmura, Shigeto

AU - Tsubota, Kazuo

AU - Amano, Shiro

AU - Kawakami, Yutaka

AU - Ishida, Susumu

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