Suppression of ethanol-induced apoptotic DNA fragmentation by geranylgeranylacetone in cultured guinea pig gastric mucosal cells

Tohru Mizushima, Shinji Tsutsumi, Kazuhito Rokutan, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

43 Citations (Scopus)


The purpose of this study was to elucidate the molecular mechanism of action of geranylgeranylacetone, an antiulcer drug. Treatment with ethanol for 8 hr at the optimum concentration (7.5%) caused apoptotic DNA fragmentation in cultured guinea pig gastric mucosal cells. Pretreatment of cells with geranylgeranylacetone suppressed the DNA fragmentation in a dose- dependent manner. The maximum effect was achieved at 10-6 M, at which concentration the drug was previously shown to induce heat-shock proteins. The suppression required an incubation period longer than 1 hr. Pretreatment of cells with low concentrations of ethanol also prevented DNA fragmentation.

Original languageEnglish
Pages (from-to)510-514
Number of pages5
JournalDigestive Diseases and Sciences
Issue number3
Publication statusPublished - 1999 Jan 1



  • Geranylgeranylacetone; gastric mucosal cell; apoptosis; heat-shock proteins

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

Cite this