Suppression of ethanol-induced apoptotic DNA fragmentation by geranylgeranylacetone in cultured guinea pig gastric mucosal cells

Tohru Mizushima, Shinji Tsutsumi, Kazuhito Rokutan, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The purpose of this study was to elucidate the molecular mechanism of action of geranylgeranylacetone, an antiulcer drug. Treatment with ethanol for 8 hr at the optimum concentration (7.5%) caused apoptotic DNA fragmentation in cultured guinea pig gastric mucosal cells. Pretreatment of cells with geranylgeranylacetone suppressed the DNA fragmentation in a dose- dependent manner. The maximum effect was achieved at 10-6 M, at which concentration the drug was previously shown to induce heat-shock proteins. The suppression required an incubation period longer than 1 hr. Pretreatment of cells with low concentrations of ethanol also prevented DNA fragmentation.

Original languageEnglish
Pages (from-to)510-514
Number of pages5
JournalDigestive Diseases and Sciences
Volume44
Issue number3
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

geranylgeranylacetone
DNA Fragmentation
Stomach
Guinea Pigs
Ethanol
Heat-Shock Proteins
Pharmaceutical Preparations

Keywords

  • Geranylgeranylacetone; gastric mucosal cell; apoptosis; heat-shock proteins

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Suppression of ethanol-induced apoptotic DNA fragmentation by geranylgeranylacetone in cultured guinea pig gastric mucosal cells. / Mizushima, Tohru; Tsutsumi, Shinji; Rokutan, Kazuhito; Tsuchiya, Tomofusa.

In: Digestive Diseases and Sciences, Vol. 44, No. 3, 1999, p. 510-514.

Research output: Contribution to journalArticle

Mizushima, Tohru ; Tsutsumi, Shinji ; Rokutan, Kazuhito ; Tsuchiya, Tomofusa. / Suppression of ethanol-induced apoptotic DNA fragmentation by geranylgeranylacetone in cultured guinea pig gastric mucosal cells. In: Digestive Diseases and Sciences. 1999 ; Vol. 44, No. 3. pp. 510-514.
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