TY - JOUR
T1 - Suppression of ethanol-induced apoptotic DNA fragmentation by geranylgeranylacetone in cultured guinea pig gastric mucosal cells
AU - Mizushima, Tohru
AU - Tsutsumi, Shinji
AU - Rokutan, Kazuhito
AU - Tsuchiya, Tomofusa
N1 - Funding Information:
Manuscript received July 23, 1998; revised manuscript received October 14, 1998; acce pted October 26, 1998. From the Faculty of Pharmace utical Science s, Okayama Unive r-sity, Okayaa m70-8030,5Japan; and Departmen t of Nutrition, School of Medicine, The University of Tokushima, Tokushima 770-8503, Japan. ThisworkwassupportedinpartbyGrantsinAidforScienti® c Research from the Ministry of Education, Science , Sports, and Culture, Japan. Address for reprint requests: Dr. Tohru Mizushima, Faculty of Pharmace utical Sciences, Okayama University, 1-1-1, Tsushima-naka, Okaaya m700853-, J0aap. n
PY - 1999
Y1 - 1999
N2 - The purpose of this study was to elucidate the molecular mechanism of action of geranylgeranylacetone, an antiulcer drug. Treatment with ethanol for 8 hr at the optimum concentration (7.5%) caused apoptotic DNA fragmentation in cultured guinea pig gastric mucosal cells. Pretreatment of cells with geranylgeranylacetone suppressed the DNA fragmentation in a dose- dependent manner. The maximum effect was achieved at 10-6 M, at which concentration the drug was previously shown to induce heat-shock proteins. The suppression required an incubation period longer than 1 hr. Pretreatment of cells with low concentrations of ethanol also prevented DNA fragmentation.
AB - The purpose of this study was to elucidate the molecular mechanism of action of geranylgeranylacetone, an antiulcer drug. Treatment with ethanol for 8 hr at the optimum concentration (7.5%) caused apoptotic DNA fragmentation in cultured guinea pig gastric mucosal cells. Pretreatment of cells with geranylgeranylacetone suppressed the DNA fragmentation in a dose- dependent manner. The maximum effect was achieved at 10-6 M, at which concentration the drug was previously shown to induce heat-shock proteins. The suppression required an incubation period longer than 1 hr. Pretreatment of cells with low concentrations of ethanol also prevented DNA fragmentation.
KW - Geranylgeranylacetone; gastric mucosal cell; apoptosis; heat-shock proteins
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U2 - 10.1023/A:1026692920848
DO - 10.1023/A:1026692920848
M3 - Article
C2 - 10080142
AN - SCOPUS:0033027343
SN - 0002-9211
VL - 44
SP - 510
EP - 514
JO - American Journal of Digestive Diseases
JF - American Journal of Digestive Diseases
IS - 3
ER -