Abstract
We have previously established and characterized two monoclonal antibodies, 8F11 and 20A11, that recognize an M, 44,000 membrane glycoprotein of metastatic marine colon 26 cells. Both monoclonal antibodies inhibit platelet aggregation induced by the tumor cells in vitro. In this report, the inhibitory effect of 8F11 on lung colonization of i.v.-inoculated tumor cells was examined. The i.v. administration of 8F11 suppressed lung colonization of NL-17, a highly metastatic variant of colon 26. Inhibition of NL-17 lung colonization by 8F11 was dose dependent with a maximum of 80% inhibition at a dose of 800 μg 8F11/ mouse. 8F11 did not inhibit metastases at doses lower than 100 μg/ mouse. Inhibition of pulmonary metastases by 8F11 was greatest when the antibody was administered 2 h before tumor inoculation. The effect. was diminished when the antibody was given 2 h after tumor inoculation. The pulmonary retention of i.v.-inoculated radiolabeled NL-17 cells was decreased by 8F11. F(ab′)2 fragments of 8F11 also effectively inhibited lung colonization by NL-17 cells, suggesting that mechanisms unrelated to immune-mediated destruction are involved. These results indicate that the monoclonal antibody 8F11 suppresses the lung colonization of NL-17 cells by interfering with the initial arrest of tumor cells in the lung vasculature through the inhibition of tumor cell-platelet interaction.
| Original language | English |
|---|---|
| Pages (from-to) | 921-925 |
| Number of pages | 5 |
| Journal | Cancer Research |
| Volume | 51 |
| Issue number | 3 |
| Publication status | Published - 1991 Feb 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Suppression of experimental lung colonization of a metastatic variant of murine colon adenocarcinoma 26 by a monoclonal antibody 8F11 inhibiting tumor cell-induced platelet aggregation. / Sugimoto, Yoshikazu; Watanabe, Masahiko; Oh-hara, Tomoko; Sato, Shigeo; Isoe, Toshiyuki; Tsuruo, Takashi.
In: Cancer Research, Vol. 51, No. 3, 01.02.1991, p. 921-925.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Suppression of experimental lung colonization of a metastatic variant of murine colon adenocarcinoma 26 by a monoclonal antibody 8F11 inhibiting tumor cell-induced platelet aggregation
AU - Sugimoto, Yoshikazu
AU - Watanabe, Masahiko
AU - Oh-hara, Tomoko
AU - Sato, Shigeo
AU - Isoe, Toshiyuki
AU - Tsuruo, Takashi
PY - 1991/2/1
Y1 - 1991/2/1
N2 - We have previously established and characterized two monoclonal antibodies, 8F11 and 20A11, that recognize an M, 44,000 membrane glycoprotein of metastatic marine colon 26 cells. Both monoclonal antibodies inhibit platelet aggregation induced by the tumor cells in vitro. In this report, the inhibitory effect of 8F11 on lung colonization of i.v.-inoculated tumor cells was examined. The i.v. administration of 8F11 suppressed lung colonization of NL-17, a highly metastatic variant of colon 26. Inhibition of NL-17 lung colonization by 8F11 was dose dependent with a maximum of 80% inhibition at a dose of 800 μg 8F11/ mouse. 8F11 did not inhibit metastases at doses lower than 100 μg/ mouse. Inhibition of pulmonary metastases by 8F11 was greatest when the antibody was administered 2 h before tumor inoculation. The effect. was diminished when the antibody was given 2 h after tumor inoculation. The pulmonary retention of i.v.-inoculated radiolabeled NL-17 cells was decreased by 8F11. F(ab′)2 fragments of 8F11 also effectively inhibited lung colonization by NL-17 cells, suggesting that mechanisms unrelated to immune-mediated destruction are involved. These results indicate that the monoclonal antibody 8F11 suppresses the lung colonization of NL-17 cells by interfering with the initial arrest of tumor cells in the lung vasculature through the inhibition of tumor cell-platelet interaction.
AB - We have previously established and characterized two monoclonal antibodies, 8F11 and 20A11, that recognize an M, 44,000 membrane glycoprotein of metastatic marine colon 26 cells. Both monoclonal antibodies inhibit platelet aggregation induced by the tumor cells in vitro. In this report, the inhibitory effect of 8F11 on lung colonization of i.v.-inoculated tumor cells was examined. The i.v. administration of 8F11 suppressed lung colonization of NL-17, a highly metastatic variant of colon 26. Inhibition of NL-17 lung colonization by 8F11 was dose dependent with a maximum of 80% inhibition at a dose of 800 μg 8F11/ mouse. 8F11 did not inhibit metastases at doses lower than 100 μg/ mouse. Inhibition of pulmonary metastases by 8F11 was greatest when the antibody was administered 2 h before tumor inoculation. The effect. was diminished when the antibody was given 2 h after tumor inoculation. The pulmonary retention of i.v.-inoculated radiolabeled NL-17 cells was decreased by 8F11. F(ab′)2 fragments of 8F11 also effectively inhibited lung colonization by NL-17 cells, suggesting that mechanisms unrelated to immune-mediated destruction are involved. These results indicate that the monoclonal antibody 8F11 suppresses the lung colonization of NL-17 cells by interfering with the initial arrest of tumor cells in the lung vasculature through the inhibition of tumor cell-platelet interaction.
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M3 - Article
VL - 51
SP - 921
EP - 925
JO - Cancer Research
JF - Cancer Research
SN - 0008-5472
IS - 3
ER -