Suppression of glial tumor growth by expression of glial fibrillary acidic protein

Masahiro Toda, Masayuki Miura, Hiroaki Asou, Ichiro Sugiyama, Takeshi Kawase, Keiichi Uyemura

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We examined the effect of expression of glial fibrillary acidic protein (GFAP) on the tumor growth of astrocytoma in vivo. When rat astrocytoma C6 cells were injected subcutaneously in athymic mice, the cells produced tumors that grew rapidly. The tumor growth of C6 cells transfected with GFAP cDNA was significantly reduced compared to that of control NeoC6 cells transfected only with the neomycin resistant gene. After implantation of C6 cells transfected with mutated GFAP cDNA at the phosphorylation sites, the tumor growth was suppressed similar to that of the wild GFAP transfectants. To determine whether the cell growth suppression by GFAP is specific for astroglial cells, we assessed the effect of GFAP on the cell growth of an L cell of fibroblast origin in vitro. By GFAP cDNA transfection, L cells showed morphological changes, but the cell growth was not reduced. These results suggest that GFAP is a critical regulator of the tumor growth of astrocytoma.

Original languageEnglish
Pages (from-to)339-343
Number of pages5
JournalNeurochemical Research
Volume24
Issue number2
DOIs
Publication statusPublished - 1999 Feb 13

Keywords

  • Astrocytoma
  • Fibroblast
  • GFAP
  • Mutation
  • Tumor suppression

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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