Suppression of in vitro production of anti-U1-ribonucleoprotein antibody by monoclonal anti-idiotypic antibody to anti-U1-ribonucleoprotein antibody

J. Koide, Tsutomu Takeuchi, O. Hosono, M. Takano, T. Abe

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We produced monoclonal anti-idiotypic antibody against immunoaffinity purified anti-U1-ribonucleoprotein (RNP) antibody from a patient (K.T.), by the cell fusion procedure. The specificity of monoclonal anti-idiotypic antibody (IgG1, κ) was determined by inhibition studies. With the monoclonal anti-idiotypic antibody, cross-reactive idiotypes on anti-U1-RNP antibodies from unrelated patients with anti-U1-RNP antibody was detected in 57% of the samples. The anti-idiotypic antibody specifically suppressed the in vitro production of anti-U1-RNP antibody by lymphocytes from the patient K.T., and unrelated patients with a cross-reactive idiotype, in whom idiotype-reactive T cells were demonstrated. The results indicate that anti-idiotypic antibody may modulate the regulation of in vitro anti-U1-RNP antibody production.

Original languageEnglish
Pages (from-to)687-696
Number of pages10
JournalScandinavian Journal of Immunology
Volume28
Issue number6
Publication statusPublished - 1988
Externally publishedYes

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Ribonucleoproteins
Anti-Idiotypic Antibodies
Monoclonal Antibodies
Antibodies
Cell Fusion
Antibody Formation
Immunoglobulin G
In Vitro Techniques
Lymphocytes
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Suppression of in vitro production of anti-U1-ribonucleoprotein antibody by monoclonal anti-idiotypic antibody to anti-U1-ribonucleoprotein antibody. / Koide, J.; Takeuchi, Tsutomu; Hosono, O.; Takano, M.; Abe, T.

In: Scandinavian Journal of Immunology, Vol. 28, No. 6, 1988, p. 687-696.

Research output: Contribution to journalArticle

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AU - Hosono, O.

AU - Takano, M.

AU - Abe, T.

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