Suppressive effect of LD78 on the proliferation of human hemopoietic progenitors

Hiroko Shiozaki, Toshio Ide, Junji Nakao, Takayuki Imamura, Mitsuru Nakamura, Kazunori Shimada, Yasusada Miura, Toshio Suda

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

LD78 is a cDNA newly isolated from human stimulated tonsillar lymphocytes. The expression of LD78 is related to inflammatory responses and its structure has a homology with macrophage inflammatory protein 1-α, which is known to have an inhibitory effect on murine CFU-S. Using a colony assay technique, we examined the effects of LD78 on human hemopoietic progenitors. The addition of doses of 100 ng/ml or more of LD78 suppressed the colony formation of KMT-2, a factor-dependent myelomonocytic cell line established from cord blood cells; this suppressive activity was neutralized by the addition of antibody against LD78. The same doses of LD78 suppressed the formation of neutrophil, macrophage, and megakaryocytic colonies which were supported by human interleukin-3 and erythropoietin; however, LD78 did not affect colony formation by either non-phagocytic mononuclear cells or sorted CD34+ cells. The conditioned medium of KMT-2 cells or peripheral blood mononuclear cells cultured with LD78 suppressed colony formation by CD34+ cells. From these findings, it is suggested that LD78 affects phagocytic cells and induces factors that are inhibitory for hemopoiesis. We consider LD78 to be a new cytokine that plays an inhibitory role in hemopoiesis.

Original languageEnglish
Pages (from-to)499-504
Number of pages6
JournalJapanese Journal of Cancer Research
Volume83
Issue number5
Publication statusPublished - 1992 May

Fingerprint

Blood Cells
Macrophage Inflammatory Proteins
Interleukin-3
Phagocytes
Conditioned Culture Medium
Erythropoietin
Fetal Blood
Neutrophils
Complementary DNA
Macrophages
Lymphocytes
Cytokines
Cell Line
Antibodies

Keywords

  • CD34
  • Hemopoietic progenitor
  • LD78

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Shiozaki, H., Ide, T., Nakao, J., Imamura, T., Nakamura, M., Shimada, K., ... Suda, T. (1992). Suppressive effect of LD78 on the proliferation of human hemopoietic progenitors. Japanese Journal of Cancer Research, 83(5), 499-504.

Suppressive effect of LD78 on the proliferation of human hemopoietic progenitors. / Shiozaki, Hiroko; Ide, Toshio; Nakao, Junji; Imamura, Takayuki; Nakamura, Mitsuru; Shimada, Kazunori; Miura, Yasusada; Suda, Toshio.

In: Japanese Journal of Cancer Research, Vol. 83, No. 5, 05.1992, p. 499-504.

Research output: Contribution to journalArticle

Shiozaki, H, Ide, T, Nakao, J, Imamura, T, Nakamura, M, Shimada, K, Miura, Y & Suda, T 1992, 'Suppressive effect of LD78 on the proliferation of human hemopoietic progenitors', Japanese Journal of Cancer Research, vol. 83, no. 5, pp. 499-504.
Shiozaki H, Ide T, Nakao J, Imamura T, Nakamura M, Shimada K et al. Suppressive effect of LD78 on the proliferation of human hemopoietic progenitors. Japanese Journal of Cancer Research. 1992 May;83(5):499-504.
Shiozaki, Hiroko ; Ide, Toshio ; Nakao, Junji ; Imamura, Takayuki ; Nakamura, Mitsuru ; Shimada, Kazunori ; Miura, Yasusada ; Suda, Toshio. / Suppressive effect of LD78 on the proliferation of human hemopoietic progenitors. In: Japanese Journal of Cancer Research. 1992 ; Vol. 83, No. 5. pp. 499-504.
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