Suppressive effect of locally produced interleukin-10 on respiratory syncytial virus infection

Yan Ruan; Yoshitaka Okamoto; Zensei Matsuzaki; Shuichiro Endo; Tomokazu Matsuoka; Tadashi Kohno; Hideaki Chazono; Ito Eiko; Kazuo Tsubota; Ichiro Saito

doi:10.1046/j.1365-2567.2001.01318.x

Suppressive effect of locally produced interleukin-10 on respiratory syncytial virus infection

Yan Ruan, Yoshitaka Okamoto, Zensei Matsuzaki, Shuichiro Endo, Tomokazu Matsuoka, Tadashi Kohno, Hideaki Chazono, Ito Eiko, Kazuo Tsubota, Ichiro Saito

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Interleukin (IL)-10 is known to be a multifunctional cytokine. This study was designed to evaluate the role of IL-10 during respiratory syncytial virus (RSV) infection using a C57BL/6 transgenic (TG) mouse model in which the expression of murine IL-10 cDNA was regulated by a human salivary amylase promoter (IL-10 TG mice). These mice expressed a large amount of IL-10 in the nasal mucosa and in salivary glands. Viral replication in the respiratory tract after intranasal infection with RSV was suppressed significantly in IL-10 TG mice compared to non-transgenic controls. This suppression was IL-10 specific, because it was prevented by treating mice with neutralizing anti-IL-10 antibodies. We also found that IL-10-stimulated T cells displayed cytotoxic activity against infected murine nasal epithelial cells. Previous data indicated that IL-10 induces Fas ligand (L) expression on mouse T cells. Taken together, these data suggest that Fas/Fas L mediated cytotoxicity is involved in the suppression of RSV replication observed in IL-10 TG mice after intranasal infection.

Original language	English
Pages (from-to)	355-360
Number of pages	6
Journal	Immunology
Volume	104
Issue number	3
DOIs	https://doi.org/10.1046/j.1365-2567.2001.01318.x
Publication status	Published - 2001
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1046/j.1365-2567.2001.01318.x

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title = "Suppressive effect of locally produced interleukin-10 on respiratory syncytial virus infection",

abstract = "Interleukin (IL)-10 is known to be a multifunctional cytokine. This study was designed to evaluate the role of IL-10 during respiratory syncytial virus (RSV) infection using a C57BL/6 transgenic (TG) mouse model in which the expression of murine IL-10 cDNA was regulated by a human salivary amylase promoter (IL-10 TG mice). These mice expressed a large amount of IL-10 in the nasal mucosa and in salivary glands. Viral replication in the respiratory tract after intranasal infection with RSV was suppressed significantly in IL-10 TG mice compared to non-transgenic controls. This suppression was IL-10 specific, because it was prevented by treating mice with neutralizing anti-IL-10 antibodies. We also found that IL-10-stimulated T cells displayed cytotoxic activity against infected murine nasal epithelial cells. Previous data indicated that IL-10 induces Fas ligand (L) expression on mouse T cells. Taken together, these data suggest that Fas/Fas L mediated cytotoxicity is involved in the suppression of RSV replication observed in IL-10 TG mice after intranasal infection.",

author = "Yan Ruan and Yoshitaka Okamoto and Zensei Matsuzaki and Shuichiro Endo and Tomokazu Matsuoka and Tadashi Kohno and Hideaki Chazono and Ito Eiko and Kazuo Tsubota and Ichiro Saito",

year = "2001",

doi = "10.1046/j.1365-2567.2001.01318.x",

language = "English",

volume = "104",

pages = "355--360",

journal = "Immunology",

issn = "0019-2805",

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T1 - Suppressive effect of locally produced interleukin-10 on respiratory syncytial virus infection

AU - Ruan, Yan

AU - Okamoto, Yoshitaka

AU - Matsuzaki, Zensei

AU - Endo, Shuichiro

AU - Matsuoka, Tomokazu

AU - Kohno, Tadashi

AU - Chazono, Hideaki

AU - Eiko, Ito

AU - Tsubota, Kazuo

AU - Saito, Ichiro

PY - 2001

Y1 - 2001

N2 - Interleukin (IL)-10 is known to be a multifunctional cytokine. This study was designed to evaluate the role of IL-10 during respiratory syncytial virus (RSV) infection using a C57BL/6 transgenic (TG) mouse model in which the expression of murine IL-10 cDNA was regulated by a human salivary amylase promoter (IL-10 TG mice). These mice expressed a large amount of IL-10 in the nasal mucosa and in salivary glands. Viral replication in the respiratory tract after intranasal infection with RSV was suppressed significantly in IL-10 TG mice compared to non-transgenic controls. This suppression was IL-10 specific, because it was prevented by treating mice with neutralizing anti-IL-10 antibodies. We also found that IL-10-stimulated T cells displayed cytotoxic activity against infected murine nasal epithelial cells. Previous data indicated that IL-10 induces Fas ligand (L) expression on mouse T cells. Taken together, these data suggest that Fas/Fas L mediated cytotoxicity is involved in the suppression of RSV replication observed in IL-10 TG mice after intranasal infection.

AB - Interleukin (IL)-10 is known to be a multifunctional cytokine. This study was designed to evaluate the role of IL-10 during respiratory syncytial virus (RSV) infection using a C57BL/6 transgenic (TG) mouse model in which the expression of murine IL-10 cDNA was regulated by a human salivary amylase promoter (IL-10 TG mice). These mice expressed a large amount of IL-10 in the nasal mucosa and in salivary glands. Viral replication in the respiratory tract after intranasal infection with RSV was suppressed significantly in IL-10 TG mice compared to non-transgenic controls. This suppression was IL-10 specific, because it was prevented by treating mice with neutralizing anti-IL-10 antibodies. We also found that IL-10-stimulated T cells displayed cytotoxic activity against infected murine nasal epithelial cells. Previous data indicated that IL-10 induces Fas ligand (L) expression on mouse T cells. Taken together, these data suggest that Fas/Fas L mediated cytotoxicity is involved in the suppression of RSV replication observed in IL-10 TG mice after intranasal infection.

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AN - SCOPUS:0035158887

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SP - 355

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JO - Immunology

JF - Immunology

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ER -

Suppressive effect of locally produced interleukin-10 on respiratory syncytial virus infection

Abstract

ASJC Scopus subject areas

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