Suppressive effect of locally produced interleukin-10 on respiratory syncytial virus infection

Yan Ruan, Yoshitaka Okamoto, Zensei Matsuzaki, Shuichiro Endo, Tomokazu Matsuoka, Tadashi Kohno, Hideaki Chazono, Ito Eiko, Kazuo Tsubota, Ichiro Saito

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Interleukin (IL)-10 is known to be a multifunctional cytokine. This study was designed to evaluate the role of IL-10 during respiratory syncytial virus (RSV) infection using a C57BL/6 transgenic (TG) mouse model in which the expression of murine IL-10 cDNA was regulated by a human salivary amylase promoter (IL-10 TG mice). These mice expressed a large amount of IL-10 in the nasal mucosa and in salivary glands. Viral replication in the respiratory tract after intranasal infection with RSV was suppressed significantly in IL-10 TG mice compared to non-transgenic controls. This suppression was IL-10 specific, because it was prevented by treating mice with neutralizing anti-IL-10 antibodies. We also found that IL-10-stimulated T cells displayed cytotoxic activity against infected murine nasal epithelial cells. Previous data indicated that IL-10 induces Fas ligand (L) expression on mouse T cells. Taken together, these data suggest that Fas/Fas L mediated cytotoxicity is involved in the suppression of RSV replication observed in IL-10 TG mice after intranasal infection.

Original languageEnglish
Pages (from-to)355-360
Number of pages6
JournalImmunology
Volume104
Issue number3
DOIs
Publication statusPublished - 2001
Externally publishedYes

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Respiratory Syncytial Virus Infections
Interleukin-10
Transgenic Mice
T-Lymphocytes
Fas Ligand Protein
Respiratory Syncytial Viruses
Nasal Mucosa
Amylases
Virus Replication
Salivary Glands
Nose
Respiratory System
Complementary DNA
Epithelial Cells

ASJC Scopus subject areas

  • Immunology

Cite this

Ruan, Y., Okamoto, Y., Matsuzaki, Z., Endo, S., Matsuoka, T., Kohno, T., ... Saito, I. (2001). Suppressive effect of locally produced interleukin-10 on respiratory syncytial virus infection. Immunology, 104(3), 355-360. https://doi.org/10.1046/j.1365-2567.2001.01318.x

Suppressive effect of locally produced interleukin-10 on respiratory syncytial virus infection. / Ruan, Yan; Okamoto, Yoshitaka; Matsuzaki, Zensei; Endo, Shuichiro; Matsuoka, Tomokazu; Kohno, Tadashi; Chazono, Hideaki; Eiko, Ito; Tsubota, Kazuo; Saito, Ichiro.

In: Immunology, Vol. 104, No. 3, 2001, p. 355-360.

Research output: Contribution to journalArticle

Ruan, Y, Okamoto, Y, Matsuzaki, Z, Endo, S, Matsuoka, T, Kohno, T, Chazono, H, Eiko, I, Tsubota, K & Saito, I 2001, 'Suppressive effect of locally produced interleukin-10 on respiratory syncytial virus infection', Immunology, vol. 104, no. 3, pp. 355-360. https://doi.org/10.1046/j.1365-2567.2001.01318.x
Ruan, Yan ; Okamoto, Yoshitaka ; Matsuzaki, Zensei ; Endo, Shuichiro ; Matsuoka, Tomokazu ; Kohno, Tadashi ; Chazono, Hideaki ; Eiko, Ito ; Tsubota, Kazuo ; Saito, Ichiro. / Suppressive effect of locally produced interleukin-10 on respiratory syncytial virus infection. In: Immunology. 2001 ; Vol. 104, No. 3. pp. 355-360.
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